Anticorps Polyclonal de lapin anti-SMPDL3B

• Phare
• Validé par KD/KO

SMPDL3B Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

Avis (1)

N° de cat : 16552-1-AP

Synonymes

ASM like phosphodiesterase 3b, ASML3B, SMPDL3B

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Galerie de données de validation

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  WB analysis using 16552-1-AP

  COLO 320 cells lysates were subjected to SDS PAGE followed by western blot with 16552-1-AP (SMPDL3B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 16552-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16552-1-AP (SMPDL3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 16552-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16552-1-AP (SMPDL3B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 16552-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16552-1-AP (SMPDL3B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 16552-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16552-1-AP (SMPDL3B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 16552-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16552-1-AP (SMPDL3B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules COLO 320, cellules HEK-293
• Résultats positifs en IHC: tissu rénal humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

16552-1-AP cible SMPDL3B dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SMPDL3B Protéine recombinante Ag9535
• Nom complet: sphingomyelin phosphodiesterase, acid-like 3B
• Masse moléculaire calculée: 373aa,42 kDa; 455aa,51 kDa
• Poids moléculaire observé: 42 kDa, 50-65 kDa
• Numéro d’acquisition GenBank: BC014444
• Symbole du gène: SMPDL3B
• Identification du gène (NCBI): 27293
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SMPDL3B is lipid-modulating phosphodiesterase. Active on the surface of macrophages and dendritic cells and strongly influences macrophage lipid composition and membrane fluidity. SMPDL3B acts as a negative regulator of Toll-like receptor signaling.It lacks activity with phosphocholine-containing lipids, but can cleave CDP-choline, and can release phosphate from ATP and ADP in vitro (PMID: 26095358). SMPDL3B can be detected 60-70 kDa by N-glycosylation modification (PMID: 26095358, PMID: 31217420).

Publications

Species	Application	Title
human	WB	Front Mol Biosci; SMPDL3B Predicts Poor Prognosis and Contributes to Development of Acute Myeloid Leukemia; Authors - Huiqing Qu ; KO Validated
rat	WB,IHC	Basic Clin Pharmacol Toxicol; PMID: 29923306; Authors - Xin Li
mouse	WB	JHEP Rep; Macrophage-derived thrombospondin 1 promotes obesity-associated non-alcoholic fatty liver disease.; Authors - Taesik Gwag
human,pig,monkey	WB,IF	iScience; Porcine reproductive and respiratory syndrome virus upregulates SMPDL3B to promote viral replication by modulating lipid metabolism; Authors - Huan-Huan Shen ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kamal (Verified Customer) (02-15-2024)

This antibody works well for mouse liver tissues.

• Applications: Western Blot
• Primary Antibody Dilution: 1:10000
• Cell Tissue Type: Mouse liver tissue