Anticorps Polyclonal de lapin anti-Spermine synthase

• Phare
• Validé par KD/KO

Spermine synthase Polyclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(10)

Avis (1)

N° de cat : 15979-1-AP

Synonymes

SMS, EC:2.5.1.22, MRSR, SPMSY, SpS

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Galerie de données de validation

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  WB analysis of Jurkat using 15979-1-AP

  WB result of Spermine synthase antibody (15979-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Spermine synthase transfected Jurkat cells.

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  WB analysis using 15979-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15979-1-AP (Spermine synthase antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 15979-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 15979-1-AP (Spermine synthase antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 15979-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Spermine synthase antibody (15979-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of HeLa using 15979-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Spermine synthase Polyclonal antibody (15979-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules Jurkat, tissu cardiaque humain
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 9 publications below
• IHC: See 2 publications below

Informations sur le produit

15979-1-AP cible Spermine synthase dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Spermine synthase Protéine recombinante Ag8767
• Nom complet: spermine synthase
• Masse moléculaire calculée: 366 aa, 41 kDa
• Poids moléculaire observé: 41 kDa, 60 kDa
• Numéro d’acquisition GenBank: BC009898
• Symbole du gène: SMS
• Identification du gène (NCBI): 6611
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB,IHC	Sci Rep; Predicting lung adenocarcinoma prognosis, immune escape, and pharmacomic profile from arginine and proline-related genes; Authors - Ziqiang Wang
human	WB	Cells; Magnesium Supplementation Attenuates Ultraviolet-B-Induced Damage Mediated through Elevation of Polyamine Production in Human HaCaT Keratinocytes.; Authors - Shokoku Shu
human	WB	Proteomics; Changes in the proteomic profile during the differential polarization status of the human monocyte-derived macrophage THP-1 cell line.; Authors - Fan Zhang
mouse	WB	J Proteomics; Proteomic analysis revealed different responses to hypergravity of soleus and extensor digitorum longus muscles in mice.; Authors - Takashi Ohira
	IHC	Biol Reprod; Progesterone and interferon tau regulate expression of polyamine enzymes during the ovine peri-implantation period.; Authors - Katherine M Halloran
mouse	WB	Alzheimers Res Ther; Spermidine/spermine-N1-acetyltransferase ablation impacts tauopathy-induced polyamine stress response.; Authors - Leslie A Sandusky-Beltran

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hua (Verified Customer) (03-28-2025)

This antibody is working in WB with mouse liver lysate. However the signal is very weak.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse liver

Spermine synthase Antibody Western Blot validation (1:500 dilution) in Mouse liver (Cat no:15979-1-AP)

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