Anticorps Polyclonal de lapin anti-SNAPIN

SNAPIN Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

13

N° de cat : 10055-1-AP

Synonymes

SNAP associated protein, SNAP25BP, SNAPAP, SNAPIN

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Galerie de données de validation

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  WB analysis of A375 using 10055-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 10055-1-AP (SNAPIN Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 10055-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10055-1-AP (SNAPIN Antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10055-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10055-1-AP (SNAPIN Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 10055-1-AP

  IP Result of anti-SNAPIN (IP:10055-1-AP, 5ug; Detection:10055-1-AP 1:300) with mouse brain tissue lysate 4000ug.

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  IHC staining of human testis using 10055-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10055-1-AP (SNAPIN Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 10055-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10055-1-AP (SNAPIN Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human brain using 10055-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10055-1-AP (SNAPIN Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 10055-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10055-1-AP (SNAPIN Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 10055-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SNAPIN antibody (10055-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 10055-1-AP

  Immunofluorescent analysis of HepG2 cells using 10055-1-AP (SNAPIN antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 10055-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug SNAPIN antibody (10055-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: cellules A375, cellules HEK-293, tissu cérébral de rat
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu testiculaire humain, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,
• Résultats positifs en cytométrie: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 11 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

10055-1-AP cible SNAPIN dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SNAPIN Protéine recombinante Ag0101
• Nom complet: SNAP-associated protein
• Masse moléculaire calculée: 15 kDa
• Poids moléculaire observé: 15-18 kDa
• Numéro d’acquisition GenBank: BC000761
• Symbole du gène: SNAPIN
• Identification du gène (NCBI): 23557
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Snapin, a protein of relative molecular weight of 15kd, is implicated in neurotransmission by binding to SNAP-25. Snapin was enriched in neurons and exclusively located on synaptic vesicle membrane, which may be a PKA target for modulating transmitter release through the cAMP-dependent signal-transduction pathway. This anitbody can recognize the 15-18kd(Monomer) and 30-36kd(Dimer) forms of SNAPIN.

Publications

Species	Application	Title
human		J Thromb Haemost; Dysregulation of PLDN (pallidin) is a mechanism for platelet dense granule deficiency in RUNX1 haplodeficiency.; Authors - G F Mao
human	WB	Am J Hum Genet; A BLOC-1 mutation screen reveals that PLDN is mutated in Hermansky-Pudlak Syndrome type 9.; Authors - Cullinane Andrew R AR
mouse	WB	Autophagy; Carbon nanotubes, but not spherical nanoparticles, block autophagy by a shape-related targeting of lysosomes in murine macrophages.; Authors - Vanessa Cohignac
human	WB	Autophagy; HIV-1 Vpr protein impairs lysosome clearance causing SNCA/alpha-synuclein accumulation in neurons.; Authors - Maryline Santerre
human	WB,IF	Autophagy; SNAPIN is critical for lysosomal acidification and autophagosome maturation in macrophages.; Authors - Bo Shi
human	WB	J Cell Biol; LAMTOR/Ragulator is a negative regulator of Arl8b- and BORC-dependent late endosomal positioning.; Authors - Przemyslaw A Filipek

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