Anticorps Polyclonal de lapin anti-SNIP1

• Phare
• Validé par KD/KO

SNIP1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(8)

N° de cat : 14950-1-AP

Synonymes

FHA domain-containing protein SNIP1, Smad nuclear-interacting protein 1, SNIP 1

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Galerie de données de validation

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  WB analysis using 14950-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14950-1-AP (SNIP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14950-1-AP

  IP result of anti-SNIP1 (IP:14950-1-AP, 4ug; Detection:14950-1-AP 1:1000) with HeLa cells lysate 2400ug.

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  IF Staining of HeLa using 14950-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SNIP1 antibody (14950-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules U2OS, cellules HeLa
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

14950-1-AP cible SNIP1 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SNIP1 Protéine recombinante Ag6772
• Nom complet: Smad nuclear interacting protein 1
• Masse moléculaire calculée: 46 kDa
• Poids moléculaire observé: 46-50 kDa
• Numéro d’acquisition GenBank: BC027040
• Symbole du gène: SNIP1
• Identification du gène (NCBI): 79753
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Smad nuclear-interacting protein 1 (SNIP1) is a novel interacting factor of the Smad protein family that is expressed in different cell types and is involved in cell proliferation, apoptosis, and differentiation.

Publications

Species	Application	Title
human	WB,IHC,IP	Nat Commun; KMT5A-methylated SNIP1 promotes triple-negative breast cancer metastasis by activating YAP signaling.; Authors - Bo Yu ; KO Validated
human	WB	PeerJ; miR-29a-3p directly targets Smad nuclear interacting protein 1 and inhibits the migration and proliferation of cervical cancer HeLa cells.; Authors - Ying Chen ; KD Validated
human	WB,CoIP	bioRxiv; mRNA interactions with disordered regions control protein activity; Authors - Yang Luo
mouse	WB,IP	Nat Commun; SNIP1 and PRC2 coordinate cell fates of neural progenitors during brain development; Authors - Yurika Matsui
human	WB	Cell Death Differ; LncRNA BCAN-AS1 stabilizes c-Myc via N6-methyladenosine-mediated binding with SNIP1 to promote pancreatic cancer; Authors - Guandi Wu
human	WB	J Proteome Res; Quantitative Proteomics Reveal the Mechanism of MiR-138-5p Suppressing Cervical Cancer via Targeting ZNF385A; Authors - Qihang Peng

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