Anticorps Polyclonal de lapin anti-SNRPB

• Phare
• Validé par KD/KO

SNRPB Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 16807-1-AP

Synonymes

Sm protein B/B, Sm protein B/B', Small nuclear ribonucleoprotein associated proteins B and B', Small nuclear ribonucleoprotein-associated proteins B and B', SmB/B

---

Galerie de données de validation

• 

  WB analysis using 16807-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16807-1-AP (SNRPB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 16807-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16807-1-AP (SNRPB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human cervical cancer using 16807-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16807-1-AP (SNRPB antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 16807-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16807-1-AP (SNRPB antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 16807-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16807-1-AP (SNRPB antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 16807-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16807-1-AP (SNRPB antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16807-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16807-1-AP (SNRPB Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 16807-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16807-1-AP (SNRPB Antibody) at dilution of 1:50 (under 40x lens).

---

Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules K-562
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, tissu de cancer du foie humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 4 publications below
• IP: See 2 publications below

Informations sur le produit

16807-1-AP cible SNRPB dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SNRPB Protéine recombinante Ag10298
• Nom complet: small nuclear ribonucleoprotein polypeptides B and B1
• Masse moléculaire calculée: 234aa,25 kDa; 285aa,30 kDa
• Poids moléculaire observé: 25-30 kDa
• Numéro d’acquisition GenBank: BC003530
• Symbole du gène: SNRPB
• Identification du gène (NCBI): 6628
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SNRPB (Small Nuclear Ribonucleoprotein Polypeptides B and B1) is a core component of the spliceosome, a complex molecular machine that plays a critical role in pre-mRNA splicing. This process is essential for the removal of introns and the joining of exons to produce mature mRNA molecules.

Publications

Species	Application	Title
human	WB,IP	Elife; Type I and II PRMTs inversely regulate post-transcriptional intron detention through Sm and CHTOP methylation.; Authors - Maxim I Maron
human	IHC	Cell Death Dis; SNRPB promotes the tumorigenic potential of NSCLC in part by regulating RAB26.; Authors - Nianli Liu ; KO Validated
human	IHC	Ann Med; SNRPB and CEP290, predicting the prognosis of diffuse large B cell lymphoma and associated with tumour immune microenvironment; Authors - Jing Tang
mouse,human	WB,IHC	Aging (Albany NY); SNRPB promotes the progression of hepatocellular carcinoma via regulating cell cycle, oxidative stress, and ferroptosis; Authors - Xiaoyan Wang
human	WB,IHC	Oncogene; The splicing factor SNRPB promotes ovarian cancer progression through regulating aberrant exon skipping of POLA1 and BRCA2; Authors - Yingwei Li ; KD Validated
mouse	IP	Adv Sci (Weinh); PRMT5 Maintains Homeostasis of the Intestinal Epithelium by Modulating Cell Proliferation and Survival; Authors - Leilei Li

{{ptg:RelatedPrimaryAntibodies}}