Anticorps Polyclonal de lapin anti-SNRPE

SNRPE Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 20407-1-AP

Synonymes

snRNP-E, snRNP E, Sm-E, SME, Sm protein E

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Galerie de données de validation

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  WB analysis using 20407-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20407-1-AP (SNRPE antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 20407-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 20407-1-AP (SNRPE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 20407-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20407-1-AP (SNRPE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 20407-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20407-1-AP (SNRPE antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human hepatocirrhosis using 20407-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20407-1-AP (SNRPE antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 20407-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20407-1-AP (SNRPE antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse brain using 20407-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20407-1-AP (SNRPE antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu placentaire de souris, tissu cérébral humain, tissu placentaire humain
• Résultats positifs en IHC: tissu cérébral de souris, tissu de cirrhose hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

20407-1-AP cible SNRPE dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SNRPE Protéine recombinante Ag14227
• Nom complet: small nuclear ribonucleoprotein polypeptide E
• Masse moléculaire calculée: 92 aa, 11 kDa
• Poids moléculaire observé: 11 kDa
• Numéro d’acquisition GenBank: BC002639
• Symbole du gène: SNRPE
• Identification du gène (NCBI): 6635
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The snRNP E (SNRPE)protein is one of several proteins associated with the U family of small nuclear RNAs that are involved in RNA processing. It functions in the U7 snRNP complex that is involved in histone 3'-end processing and also associated with snRNP U1, U2, U4/U6 and U5. This antibody raises against the full length of human SNRPE, and can recognize two bands of SNRPE, include a 9 kDa band(isoform) and a 11kDa band(SNRPE) (PMID:23246290)

Publications

Species	Application	Title
human	IF	Am J Hum Genet; Mutations in SNRPE, which encodes a core protein of the spliceosome, cause autosomal-dominant hypotrichosis simplex.; Authors - Sandra M Pasternack
human	WB,IHC	J Pathol; A novel protein-based prognostic signature improves risk stratification to guide clinical management in early-stage lung adenocarcinoma patients.; Authors - Elena Martínez-Terroba
human	WB	Front Immunol; Multi-omics analysis and experiments uncover the function of cancer stemness in ovarian cancer and establish a machine learning-based model for predicting immunotherapy responses; Authors - Zhibing Liu ; KD Validated

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