Anticorps Polyclonal de lapin anti-SNRPN

SNRPN Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

5

Avis (1)

N° de cat : 11070-1-AP

Synonymes

HCERN3, PWCR, RT LI, SM D, Sm N, Sm protein D, Sm protein N, SMN, SNRNP N, SNRPN, SNURF SNRPN

---

Galerie de données de validation

• 

  WB analysis using 11070-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11070-1-AP (SNRPN antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 11070-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11070-1-AP (SNRPN antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 11070-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11070-1-AP (SNRPN antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SH-SY5Y using 11070-1-AP

  Immunofluorescent analysis of SH-SY5Y cells, using SNRPN antibody 11070-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

---

Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu de gliome humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IF: See 3 publications below

Informations sur le produit

11070-1-AP cible SNRPN dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: bovin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SNRPN Protéine recombinante Ag1503
• Nom complet: small nuclear ribonucleoprotein polypeptide N
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 25-30 kDa
• Numéro d’acquisition GenBank: BC010057
• Symbole du gène: SNRPN
• Identification du gène (NCBI): 6638
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SNRPN, or SNURF-SNRPN, is a bicistronic imprinted gene that encodes 2 polypeptides, the SmN splicing factor, which is involved in RNA processing, and the SNRPN upstream reading frame (SNURF) polypeptide.The SNRPN gene is transcribed exclusively from the paternally inherited chromosome and shows highest expression in brain and heart. SNRPN may play a role in etiology of Prader-Willi syndrome.

Publications

Species	Application	Title
mouse	WB	Nat Med; Targeting the histone methyltransferase G9a activates imprinted genes and improves survival of a mouse model of Prader-Willi syndrome.; Authors - Yuna Kim
human	WB	J Cell Sci; Neurochondrin interacts with the SMN protein suggesting a novel mechanism for spinal muscular atrophy pathology.; Authors - Luke W Thompson
human,mouse	WB,IF	Genes (Basel); The Diagnostic Journey of a Patient with Prader-Willi-Like Syndrome and a Unique Homozygous SNURF-SNRPN Variant; Bio-Molecular Analysis and Review of the Literature.; Authors - Karlijn Pellikaan
bovine	IF	Chin Med J (Engl); Difference of gene expression between the central and the peripheral epithelia of the bovine lens.; Authors - Ma Xuan X
human	IF	Int J Mol Sci; N-Acetylglucosamine Kinase-Small Nuclear Ribonucleoprotein Polypeptide N Interaction Promotes Axodendritic Branching in Neurons via Dynein-Mediated Microtubule Transport; Authors - Binod Timalsina

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Daniel (Verified Customer) (08-23-2022)

Antibody recognises SmN, even when expressed at low levels, but can't discriminate between SmN and its paralog SmB/B'.

• Applications: Western Blot
• Primary Antibody Dilution: 1:800
• Cell Tissue Type: HeLa cells transfected with Twin-Strep tagged SmN

SNRPN Antibody Western Blot validation (1:800 dilution) in HeLa cells transfected with Twin-Strep tagged SmN (Cat no:11070-1-AP)