Anticorps Polyclonal de lapin anti-SNX6

• Phare
• Validé par KD/KO

SNX6 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 10114-1-AP

Synonymes

MSTP010, SNX6, sorting nexin 6, TFAF2, TRAF4 associated factor 2

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Galerie de données de validation

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  WB analysis using 10114-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10114-1-AP (SNX6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 10114-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 10114-1-AP (SNX6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 10114-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10114-1-AP (SNX6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10114-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10114-1-AP (SNX6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10114-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10114-1-AP (SNX6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 10114-1-AP

  IP Result of anti-SNX6 (IP:10114-1-AP, 4ug; Detection:10114-1-AP 1:600) with HepG2 cells lysate 4000ug.

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  IHC staining of mouse brain using 10114-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10114-1-AP (SNX6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 10114-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10114-1-AP (SNX6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 10114-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 10114-1-AP (SNX6 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 10114-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 10114-1-AP (SNX6 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human prostate cancer using 10114-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 10114-1-AP (SNX6 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate cancer using 10114-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 10114-1-AP (SNX6 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human prostate cancer using 10114-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10114-1-AP (SNX6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10114-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10114-1-AP (SNX6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 10114-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SNX6 antibody (10114-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  FC experiment of RAW 264.7 using 10114-1-AP

  1X10^6 RAW 264.7 cells were stained with 0.2ug SNX6 antibody (10114-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules RAW 264.7, tissu cérébral de souris, tissu placentaire humain
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu cérébral de souris, tissu cardiaque humain, tissu de cancer de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,
• Résultats positifs en cytométrie: cellules RAW 264.7

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Informations sur le produit

10114-1-AP cible SNX6 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SNX6 Protéine recombinante Ag0168
• Nom complet: sorting nexin 6
• Masse moléculaire calculée: 34 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: BC001798
• Symbole du gène: SNX6
• Identification du gène (NCBI): 58533
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Sorting nexins (SNX) have previously been shown to regulate the cell-surface expression of the human epidermal growth factor receptor. On the basis of the predicted protein sequences, several members of this family, including SNX6, have been identified. SNX6, containing coiled-coil regions within its large C-terminal domain and is found distributed in both membrane and cytosolic fractions, typical of hydrophilic peripheral membrane proteins. The functions of SNX6 are likely to be unique to endosomes, mediated in part by interactions with SNX6-specific C-terminal sequences and membrane-associated determinants

Publications

Species	Application	Title
human	WB	Nat Cell Biol; Recycling of autophagosomal components from autolysosomes by the recycler complex.; Authors - Chuchu Zhou
human	WB,IHC	Acta Biochim Biophys Sin (Shanghai); SNX6 predicts poor prognosis and contributes to the metastasis of pancreatic cancer cells via activating epithelial-mesenchymal transition.; Authors - Pengfei Hu ; KD Validated
human	WB	Front Immunol; Zika virus modulates mitochondrial dynamics, mitophagy, and mitochondria-derived vesicles to facilitate viral replication in trophoblast cells; Authors - Jae Kyung Lee