Anticorps Polyclonal de lapin anti-SOAT2

SOAT2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 21852-1-AP

Synonymes

EC:2.3.1.26, ARGP2, Acyl-coenzyme A:cholesterol acyltransferase 2, ACAT-2, ACAT2

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Galerie de données de validation

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  WB analysis using 21852-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21852-1-AP (SOAT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 21852-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 21852-1-AP (SOAT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 21852-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21852-1-AP (SOAT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human small intestine using 21852-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 21852-1-AP (SOAT2 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human small intestine using 21852-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 21852-1-AP (SOAT2 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver using 21852-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21852-1-AP (SOAT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 21852-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SOAT2 antibody (21852-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 21852-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SOAT2 antibody (21852-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules L02
• Résultats positifs en IHC: tissu hépatique humain, tissu d'intestin grêle humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 2 publications below

Informations sur le produit

21852-1-AP cible SOAT2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SOAT2 Protéine recombinante Ag16364
• Nom complet: sterol O-acyltransferase 2
• Masse moléculaire calculée: 522 aa, 60 kDa
• Poids moléculaire observé: 46 kDa
• Numéro d’acquisition GenBank: BC096090
• Symbole du gène: SOAT2
• Identification du gène (NCBI): 8435
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SOAT2, also named as ACAT2, belongs to the membrane-bound acyltransferase family and Sterol o-acyltransferase subfamily. SOAT2 is expressed preferentially in enterocytes of the intestine and hepatocytes of the liver. The role of SOAT2 in generating esterified cholesterol (EC) that is incorporated directly into atherogenic lipoproteins has made it an attractive target in dyslipidemia management and prevention. (PMID: 29246491)

Publications

Species	Application	Title
human	IHC	Cell Death Discov; Cholesterol activates the Wnt/PCP-YAP signaling in SOAT1-targeted treatment of colon cancer.; Authors - Huanji Xu
mouse	IHC	Nat Commun; Increased SOAT2 expression in aged regulatory T cells is associated with altered cholesterol metabolism and reduced anti-tumor immunity; Authors - Mingjiong Zhang
human	WB	J Lipid Res; Impaired ApoB Secretion Triggers Enhanced Secretion of ApoE to Maintain Triglyceride Homeostasis in Hepatoma Cells; Authors - Kotomi Shinozaki ; KO Validated

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