Anticorps Monoclonal anti-SOCS3

SOCS3 Monoclonal Antibody for IF-P, FC (Intra)

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

IF-P, FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

1G2B12

N° de cat : CL594-66797

Synonymes

1G2B12, ATOD4, CIS3, CIS-3, Cytokine-inducible SH2 protein 3

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Galerie de données de validation

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  IF Staining of mouse heart using CL594-66797

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite®594 SOCS3 antibody (CL594-66797, Clone: 1G2B12 ) at dilution of 1:200.

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  IF Staining of mouse heart using CL594-66797

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite®594 SOCS3 antibody (CL594-66797, Clone: 1G2B12 ) at dilution of 1:200.

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  FC experiment of Jurkat using CL594-66797

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human SOCS3 (CL594-66797, Clone:1G2B12) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF-P: tissu cardiaque de souris,
• Résultats positifs en FC (Intra): cellules Jurkat,

Dilution recommandée

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-66797 cible SOCS3 dans les applications de IF-P, FC (Intra) et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SOCS3 Protéine recombinante Ag5386
• Nom complet: suppressor of cytokine signaling 3
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 25 kDa
• Numéro d’acquisition GenBank: BC060858
• Symbole du gène: SOCS3
• Identification du gène (NCBI): 9021
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Suppressor of cytokine signaling 3 (SOCS3) is a member of SOCS family that these proteins induced to attenuate cytokine signal transduction in response to signals from a diverse range of cytokines and growth factors. SOCS3 is a negative regulatory protein that inhibits signaling induced by IL-6 via preventing JAK mediated activation of STAT3. However SOCS3 can positively regulate the ERK-MAPK pathway, inhibit the NF-kB pathway (PMID:26429311,25035930). In addition, SOCS3 brings a great impact on immune responses by inhibiting signaling stimulus, such as LPS, type I and type II IFNs, and IL-12. SOCS3 is widely expressed in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney (PMID: 22961088). Dysregulation of SOCS3 functions can cause a variety of diseases, including allergy, autoimmune diseases, inflammation and cancer (PMID:26429311).

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