Anticorps Polyclonal de lapin anti-SOD3

SOD3 Polyclonal Antibody for IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 14316-1-AP

Synonymes

EC SOD, Extracellular superoxide dismutase, SOD3, Superoxide Dismutase 3

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Galerie de données de validation

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  WB analysis using 14316-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14316-1-AP (SOD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 14316-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14316-1-AP (SOD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of human placenta using 14316-1-AP

  IP Result of anti-SOD3 (IP:14316-1-AP, 4ug; Detection:14316-1-AP 1:1000) with human placenta tissue lysate 2800ug.

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Applications testées

• Résultats positifs en WB: échantillon d'urine humaine, tissu cardiaque humain, tissu placentaire humain, urine humaine
• Résultats positifs en IP: tissu placentaire humain

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below

Informations sur le produit

14316-1-AP cible SOD3 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SOD3 Protéine recombinante Ag5556
• Nom complet: superoxide dismutase 3, extracellular
• Masse moléculaire calculée: 26 kDa
• Poids moléculaire observé: 25-33 kDa
• Numéro d’acquisition GenBank: BC014418
• Symbole du gène: SOD3
• Identification du gène (NCBI): 6649
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SOD is located intracellular (SOD-1; cytosolic, SOD-2; mitochondrial matrix) and extracellular (SOD-3), and catalyzes the dismutation of superoxide anion to H2O2.SOD3, or EC-SOD, is the most recently characterized SOD, exists as a copper and zinc-containing tetramer, and is synthesized containing a signal peptide that directs this enzyme exclusively to extracellular spaces. SOD-3 is a Mn-containing tetrameric protein with a subunit Mr of 24-35 kDa. The activity of EC-SOD is dependent on its ability to form a tetramer of 135 kDa.

Publications

Species	Application	Title
mouse,human	WB	Br J Pharmacol; Inhibition of DNA methylation derepresses PPARγ and attenuates pulmonary fibrosis.; Authors - Ai Wei
human	WB	Transl Pediatr; Identification and evaluation of fructose-bisphosphate aldolase B as a potential diagnostic biomarker in choledochal cysts patients: a quantitative proteomic analysis.; Authors - Gedong Ming
human,rat	WB	Toxicol Appl Pharmacol; (-)-Epicatechin ameliorates cigarette smoke-induced lung inflammation via inhibiting ROS/NLRP3 inflammasome pathway in rats with COPD.; Authors - Xue Tian
human	WB	Clin Immunol; Oxidative stress is involved in immunosuppression and macrophage regulation in glioblastoma; Authors - Xisong Liang ; KD Validated