Anticorps Polyclonal de lapin anti-SORCS1

SORCS1 Polyclonal Antibody for FC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, FC,ELISA

Conjugaison

Non conjugué

N° de cat : 18839-1-AP

Synonymes

hSorCS, SORCS1

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Galerie de données de validation

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  WB analysis of Y79 using 18839-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 18839-1-AP (SORCS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 18839-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18839-1-AP (SORCS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 18839-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18839-1-AP (SORCS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 18839-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 18839-1-AP (SORCS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  FC experiment of HeLa using 18839-1-AP

  1X10^6 HeLa cells were stained with 0.2ug SORCS1 antibody (18839-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

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Applications testées

• Résultats positifs en WB: cellules Y79, cellules HEK-293, tissu cérébral de souris
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

18839-1-AP cible SORCS1 dans les applications de WB, FC,ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: sortilin-related VPS10 domain containing receptor 1
• Masse moléculaire calculée: 1168 aa, 130 kDa
• Poids moléculaire observé: 130 kDa, 60-80 kDa
• Numéro d’acquisition GenBank: BC131597
• Symbole du gène: SORCS1
• Identification du gène (NCBI): 114815
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SorCS1 is a 130 kDa type I transmembrane protein, belonging to Vps10p-domain (Vps10p-D) receptor family that comprises an N-terminal Vps10p-D, a leucine-rich domain, a single transmembrane domain, and a short cytoplasmic domain. SorCS1 is synthesized as a proprotein, which is converted to the mature receptor in late Golgi compartments by cleavage of the N-terminal propeptide. Alternative splicing of human SorCS1 results in four isoforms with different cytoplasmic domains and differential expression in tissues. This antibody was generated against the C-terminal region of human SorCS1 and recognizes various forms of SorCS1. Multiple bands around 100-130 kDa as well as 60-80 kDa have been detected in Y79 cells by western blot assay.