Anticorps Polyclonal de lapin anti-SOS1

SOS1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 55041-1-AP

Synonymes

GF1, GGF1, GINGF, NS4, Son of sevenless homolog 1, SOS 1, SOS1

---

Galerie de données de validation

• 

  WB analysis using 55041-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55041-1-AP (SOS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 55041-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55041-1-AP (SOS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 55041-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55041-1-AP (SOS1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 55041-1-AP

  IP result of anti-SOS1 (IP:55041-1-AP, 4ug; Detection:55041-1-AP 1:500) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of mouse colon using 55041-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 55041-1-AP (SOS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 55041-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 55041-1-AP (SOS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 55041-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55041-1-AP (SOS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 55041-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SOS1 antibody (55041-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules K-562, cellules SKOV-3, cellules THP-1, tissu cérébral de souris
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: tissu de côlon de souris, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IF: See 1 publications below

Informations sur le produit

55041-1-AP cible SOS1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: son of sevenless homolog 1 (Drosophila)
• Masse moléculaire calculée: 152 kDa
• Poids moléculaire observé: 152 kDa
• Numéro d’acquisition GenBank: NM_005633
• Symbole du gène: SOS1
• Identification du gène (NCBI): 6654
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SOS1 promotes the exchange of Ras-bound GDP by GTP. EPS8, E3B1 and SOS1 form a tricomplex in vivo that exhibits RAC-specific guanine nucleotide exchange factor activity in vitro. Defects in SOS1 are the cause of gingival fibromatosis 1 (GGF1). Defects in SOS1 are the cause of Noonan syndrome type 4 (NS4). The antibody is specific to SOS1.

Publications

Species	Application	Title
human	WB	Cells; Determination of WWOX Function in Modulating Cellular Pathways Activated by AP-2α and AP-2γ Transcription Factors in Bladder Cancer.; Authors - Damian Kołat
human	WB	Pathol Res Pract; KBTBD2 promotes proliferation and migration of gastric cancer via activating EGFR signaling pathway; Authors - Jishuang Ding
human	WB	Cancers (Basel); Lactate Oxidase Disrupts Lactate-Activated RAS and PI3K Oncogenic Signaling; Authors - Chandler R Keller
human	WB,IF	Adv Sci (Weinh); Osteoblast-Derived ECM1 Promotes Anti-Androgen Resistance in Bone Metastatic Prostate Cancer; Authors - Xinwen Wang
human	WB	J Transl Med; F-box protein FBXO21 overexpression inhibits the proliferation and metastasis of clear cell renal cell carcinoma and is closely related to the CREB pathway and tumor immune cell infiltration; Authors - Wuping Yang
human	WB	Sci Adv; HPIP is an essential scaffolding protein running through the EGFR-RAS-ERK pathway and drives tumorigenesis; Authors - Qiwei Jiang

{{ptg:RelatedPrimaryAntibodies}}