Anticorps Polyclonal de lapin anti-SOX12

• Phare
• Validé par KD/KO

SOX12 Polyclonal Antibody for WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, RIP, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 23939-1-AP

Synonymes

Protein SOX 22, SOX12, SOX22, Transcription factor SOX 12

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Galerie de données de validation

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  WB analysis of mouse testis using 23939-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 23939-1-AP (SOX12 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse brain using 23939-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 23939-1-AP (SOX12 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of BxPC-3 using 23939-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 23939-1-AP (SOX12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 23939-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 23939-1-AP (SOX12 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, cellules BxPC-3, tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 8 publications below
• RIP: See 1 publications below

Informations sur le produit

23939-1-AP cible SOX12 dans les applications de WB, RIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SOX12 Protéine recombinante Ag21043
• Nom complet: SRY (sex determining region Y)-box 12
• Masse moléculaire calculée: 315 aa, 34 kDa
• Poids moléculaire observé: 35-40 kDa
• Numéro d’acquisition GenBank: BC067361
• Symbole du gène: SOX12
• Identification du gène (NCBI): 6666
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Mol Ther Nucleic Acids; lncRNA PRR34-AS1 promotes HCC development via modulating Wnt/β-catenin pathway by absorbing miR-296-5p and upregulating E2F2 and SOX12.; Authors - Minzhen Qin
human	WB	Cancer Gene Ther; LncRNA UCA1 promotes SOX12 expression in breast cancer by regulating m6A modification of miR-375 by METTL14 through DNA methylation.; Authors - Chengpeng Zhao
human	WB	Exp Cell Res; LncRNA LINC00680 promotes lung adenocarcinoma growth via binding to GATA6 and canceling GATA6-mediated suppression of SOX12 expression.; Authors - Xiaojuan Sun ; KD Validated
human	WB	Exp Cell Res; SOX12 promotes the growth of multiple myeloma cells by enhancing Wnt/β-catenin signaling.; Authors - Ying Gao ; KD Validated
human	WB,RIP	Environ Toxicol; Long noncoding RNA DUXAP10 promotes the stemness of glioma cells by recruiting HuR to enhance Sox12 mRNA stability.; Authors - Bolin Wu ; KD Validated
human	WB	Ann Transl Med; The lncRNA TERC promotes gastric cancer cell proliferation, migration, and invasion by sponging miR-423-5p to regulate SOX12 expression; Authors - Jianhua Wang