Anticorps Polyclonal de lapin anti-SOX5

• Phare
• Validé par KD/KO

SOX5 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 13216-1-AP

Synonymes

L SOX5, SOX5, Transcription factor SOX 5

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Galerie de données de validation

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  WB analysis using 13216-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13216-1-AP (SOX5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules A549, cellules MG U-87, cellules SH-SY5Y, tissu hépatique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

13216-1-AP cible SOX5 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SOX5 Protéine recombinante Ag4092
• Nom complet: SRY (sex determining region Y)-box 5
• Masse moléculaire calculée: 763 aa, 84 kDa
• Poids moléculaire observé: 68-70 kDa
• Numéro d’acquisition GenBank: BC029220
• Symbole du gène: SOX5
• Identification du gène (NCBI): 6660
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Sex determining region Y-box protein 5 (SOX5) is a member of the SOX family, which was identified based on the conserved homology of the high-mobility group DNA-binding motif. It has been reported that SOX5 is involved in the regulation of embryonic development, and is associated with various cancer types, including prostate cancer, glioblastoma, hepatocellular carcinoma, osteosarcoma and nasopharyngeal carcinoma. SOX5 has 5 isoforms with the molecular mass of 42, 71 and 82-84 kDa. (PMID: 30854049)

Publications

Species	Application	Title
mouse	WB	Nat Commun; VCAM1 acts in parallel with CD69 and is required for the initiation of oligodendrocyte myelination.; Authors - Yuki Miyamoto
mouse,rat	WB	Pharmacol Res; Cyclo-(Phe-Tyr) as a novel cyclic dipeptide compound alleviates ischemic/reperfusion brain injury via JUNB/JNK/NF-κB and SOX5/PI3K/AKT pathways.; Authors - Jiale Cai
human	WB,IHC	Cell Oncol (Dordr); Apatinib inhibits glycolysis by suppressing the VEGFR2/AKT1/SOX5/GLUT4 signaling pathway in ovarian cancer cells.; Authors - Lihua Chen ; KD Validated
human	WB	Phytomedicine; Transcriptomics, molecular docking, and cross-resistance profiling of nobiletin in cancer cells and synergistic interaction with doxorubicin upon SOX5 transfection.; Authors - Aveen N Adham ; KO Validated
human	WB	Lab Invest; Sox13 promotes hepatocellular carcinoma metastasis by transcriptionally activating Twist1.; Authors - Min Feng
mouse	IF	Mol Cell Biochem; Expression profiles of Sox transcription factors within the postnatal rodent testes.; Authors - Pauline Roumaud