Anticorps Polyclonal de lapin anti-SP3

SP3 Polyclonal Antibody for WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

5

Avis (1)

N° de cat : 26584-1-AP

Synonymes

SP3, Sp3 transcription factor, SPR 2, Transcription factor Sp3

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Galerie de données de validation

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  WB analysis of PC-3 using 26584-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 26584-1-AP (SP3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 26584-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 26584-1-AP (SP3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C2C12 using 26584-1-AP

  C2C12 cell were subjected to SDS PAGE followed by western blot with 26584-1-AP (SP3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules PC-3, cellule C2C12, cellules COLO 320

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 1 publications below

Informations sur le produit

26584-1-AP cible SP3 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SP3 Protéine recombinante Ag25082
• Nom complet: Sp3 transcription factor
• Masse moléculaire calculée: 781 aa, 82 kDa
• Poids moléculaire observé: 70 kDa, 115 kDa
• Numéro d’acquisition GenBank: BC126414
• Symbole du gène: SP3
• Identification du gène (NCBI): 6670
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SP3, also named as transcription factor Sp3, is a 781 amino acid protein, which contains 3 C2H2-type zinc fingers and belongs to the Sp1 C2H2-type zinc-finger protein family. SP3 localizes to the nuclear periphery and in nuclear dots when sumoylated. Some localization in PML nuclear bodies. SP3 is acetylated by histone acetyltransferase p300, deacetylated by HDACs and sumoylated on all isoforms. Sumoylated on 2 sites in longer isoforms with Lys-551 being the major site. Sumoylation at this site promotes nuclear localization to the nuclear periphery, nuclear dots and PML nuclear bodies. Sumoylation on Lys-551 represses the transactivation activity, except for the largest isoform, L-Sp3, which has little effect on transactivation. Alternate sumoylation and acetylation at Lys-551 also control transcriptional activity. There exits tow modified isoform and molecular weight of those are 70 kda and 115 kda.

Publications

Species	Application	Title
human	WB	Dig Dis Sci; Speckle-Type POZ Protein Down-Regulates Matrix Metalloproteinase 2 Expression via Sp1/PI3K/Akt Signaling Pathway in Colorectal Cancer.; Authors - Shouhua Zhang
human	WB	Biochem Biophys Res Commun; Transcriptional activation of CSTB gene expression by transcription factor Sp3; Authors - Weiyi Zhu
human	WB,IHC	Mol Cell Probes; LINC00115 regulates lung adenocarcinoma progression via sponging miR-154-3p to modulate Sp3 expression; Authors - Kexin Sun
mouse	WB	Cell Rep; LHPP-mediated inorganic pyrophosphate hydrolysis-driven lysosomal acidification in astrocytes regulates adult neurogenesis; Authors - Longze Sha
human,mouse	WB	Int Immunopharmacol; Menstrual blood-derived stem cells exosomal miR-let-7 to ameliorate pulmonary fibrosis through inhibiting ferroptosis by Sp3/HDAC2/Nrf2 signaling pathway; Authors - Lifang Sun

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Anh (Verified Customer) (07-15-2021)

clear bands, have some unspecific bands

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: mouse heart

SP3 Antibody Western Blot validation (1:500 dilution) in mouse heart (Cat no:26584-1-AP)