Anticorps Polyclonal de lapin anti-SPA17

SPA17 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 13367-1-AP

Synonymes

Cancer/testis antigen 22, CT22, SP17, SP17 1, SPA17, sperm autoantigenic protein 17, Sperm protein 17, Sperm surface protein Sp17

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Galerie de données de validation

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  WB analysis of mouse testis using 13367-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13367-1-AP (SPA17 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 13367-1-AP

  IP result of anti-SPA17 (IP:13367-1-AP, 3ug; Detection:13367-1-AP 1:400) with mouse testis tissue lysate 4800ug.

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  IHC staining of human testis using 13367-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13367-1-AP (SPA17 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 13367-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13367-1-AP (SPA17 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary tumor using 13367-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 13367-1-AP (SPA17 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary tumor using 13367-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 13367-1-AP (SPA17 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris
• Résultats positifs en IP: tissu testiculaire de souris
• Résultats positifs en IHC: tissu testiculaire humain, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

13367-1-AP cible SPA17 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SPA17 Protéine recombinante Ag4186
• Nom complet: sperm autoantigenic protein 17
• Masse moléculaire calculée: 151 aa, 17 kDa
• Poids moléculaire observé: 25 kDa
• Numéro d’acquisition GenBank: BC032457
• Symbole du gène: SPA17
• Identification du gène (NCBI): 53340
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Sperm surface protein Sp17(SPA17)is a 151 amino acids protein (17kDa), which is a sperm surface peripheral membrane protein. It is a highly conserved mammalian protein expressed in developing spermatozoa and mature spermatids, and reported to play a role in the interaction of sperm with the zona pellucida. SP17 exists as a homodimer and localizes to the head and tail of spermatozoa. SP17 has been identified as a cancer/testis antigen and is expressed in ovarian cancer and multiple myeloma. This suggests that SP17 could be suitable as a target in tumor immunotherapy.

Publications

Species	Application	Title
human	IHC	Br J Cancer; Tumour antigen expression in hepatocellular carcinoma in a low-endemic western area.; Authors - K Sideras
human	WB,IF	Front Immunol; Sperm Autoantigenic Protein 17 Predicts the Prognosis and the Immunotherapy Response of Cancers: A Pan-Cancer Analysis.; Authors - Zewei Tu
mouse	WB	Sci Rep; Testis-specific peroxiredoxin 4 variant is not absolutely required for spermatogenesis and fertility in mice.; Authors - Takujiro Homma
human	IHC	BMC Cancer; Validity and prognostic significance of sperm protein 17 as a tumor biomarker for epithelial ovarian cancer: a retrospective study.; Authors - Laurie L Brunette
human	WB	Asian J Androl; Decreased AKAP4/PKA signaling pathway in high DFI sperm affects sperm capacitation; Authors - Kun Zhang
		Nature; Targeting FOXM1 condensates reduces breast tumour growth and metastasis; Authors - Feng Xie

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