Anticorps Polyclonal de lapin anti-SPAG11A/B

SPAG11A/B Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 16770-1-AP

Synonymes

SPAG11A, Antimicrobial-like protein Bin-1b, BIN1B, EP2, He2

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Galerie de données de validation

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  WB analysis of human testis using 16770-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 16770-1-AP (SPAG11A/B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 16770-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16770-1-AP (SPAG11A/B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 16770-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16770-1-AP (SPAG11A/B antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 16770-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16770-1-AP (SPAG11A/B antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 16770-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16770-1-AP (SPAG11A/B antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 16770-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16770-1-AP (SPAG11A/B antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 16770-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16770-1-AP (SPAG11A/B antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 16770-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16770-1-AP (SPAG11A/B antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu testiculaire humain, tissu cérébral humain
• Résultats positifs en IHC: tissu testiculaire humain, tissu placentaire humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

16770-1-AP cible SPAG11A/B dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SPAG11A/B Protéine recombinante Ag9846
• Nom complet: sperm associated antigen 11A
• Masse moléculaire calculée: 123 aa, 14 kDa
• Poids moléculaire observé: 20 kDa
• Numéro d’acquisition GenBank: BC058833
• Symbole du gène: SPAG11A
• Identification du gène (NCBI): 653423
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Sperm binding proteins and their C-terminal peptides of the Sperm Associated Antigen 11 (SPAG11) family are found to play an important role in epididymal innate immunity in addition to their role in sperm maturation(PMID:16643671). SPAG11 peptides resulting due to cleavage at the furin-like proprotein convertase motif are reported in the epididymal fluid, ejaculate and on sperm and their antimicrobial activity demonstrated.SPAG11A belongs to this family and is also named as EP2,HE2. It has 4 isoforms produced by alternative splicing. This antibody recognizes SPAG11A and SPAG11B, but not SPAG11, because SPAG11 is a different transcript.

Publications

Species	Application	Title
mouse	WB,IHC	Reprod Biol Endocrinol; Sperm-associated antigen 11A is expressed exclusively in the principal cells of the mouse caput epididymis in an androgen-dependent manner.; Authors - Pujianto Dwi A DA

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