Anticorps Polyclonal de lapin anti-SPAG6

SPAG6 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(10)

N° de cat : 12462-1-AP

Synonymes

pf16, Protein PF16 homolog, Repro SA 1, SPAG6, sperm associated antigen 6, Sperm flagellar protein

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Galerie de données de validation

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  WB analysis of mouse testis using 12462-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12462-1-AP (SPAG6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 12462-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12462-1-AP (SPAG6 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 12462-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12462-1-AP (SPAG6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 12462-1-AP

  IP result of anti-SPAG6 (IP:12462-1-AP, 3ug; Detection:12462-1-AP 1:300) with mouse testis tissue lysate 4000ug.

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, cellules Jurkat, cellules PC-3
• Résultats positifs en IP: tissu testiculaire de souris

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 5 publications below

Informations sur le produit

12462-1-AP cible SPAG6 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SPAG6 Protéine recombinante Ag3130
• Nom complet: sperm associated antigen 6
• Masse moléculaire calculée: 458 aa, 50 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC030585
• Symbole du gène: SPAG6
• Identification du gène (NCBI): 9576
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Sperm-associated antigen 6 (SPAG6) is an axonemal protein ubiquitously expressed in tissues containing ciliated cells. SPAG6 is essential for sperm motility and fertility. As a novel cancer-testis antigen, SPAG6 is overexpressed in myeloid malignancies. Recently, SPAG6 has been found to negatively regulate neuronal migration during mouse brain development.

Publications

Species	Application	Title
human	IF	Front Endocrinol (Lausanne); Novel Loss-of-Function Mutations in DNAH1 Displayed Different Phenotypic Spectrum in Humans and Mice.; Authors - Ranjha Khan
mouse	WB, IF	J Mol Neurosci; Spag6 Negatively Regulates Neuronal Migration During Mouse Brain Development.; Authors - Runchuan Yan
human	WB,IHC	Front Pharmacol; SPAG6 overexpression decreases the pro-apoptotic effect of daunorubicin in acute myeloid leukemia cells through the ROS/JNK MAPK axis in a GSTP1-dependent manner; Authors - Jie Luo
human	IF	Asian J Androl; Novel homozygous SPAG17 variants cause human male infertility through multiple morphological abnormalities of spermatozoal flagella related to axonemal microtubule doublets; Authors - Tao Liu
human	WB,IHC	Int J Lab Hematol; High Expression of SPAG6 Acts as a Pro-Tumor Factor and Associated With Poor Prognosis in Acute Myeloid Leukemia; Authors - Jie Luo
human	WB	Ecotoxicol Environ Saf; Microcystin leucine arginine induces human sperm damage: Involvement of the Ca2+/CaMKKβ/AMPK pathway; Authors - Xing Guo

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