Anticorps Polyclonal de lapin anti-SPATA19

SPATA19 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 16656-1-AP

Synonymes

SPAS1, SPATA19, Spergen 1, spergen1, spermatogenesis associated 19

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Galerie de données de validation

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  WB analysis of human testis using 16656-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 16656-1-AP (SPATA19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 16656-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16656-1-AP (SPATA19 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat testis using 16656-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 16656-1-AP (SPATA19 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 16656-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 16656-1-AP (SPATA19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 16656-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16656-1-AP (SPATA19 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 16656-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16656-1-AP (SPATA19 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 16656-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16656-1-AP (SPATA19 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 16656-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16656-1-AP (SPATA19 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver using 16656-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16656-1-AP (SPATA19 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 16656-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16656-1-AP (SPATA19 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 16656-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16656-1-AP (SPATA19 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 16656-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16656-1-AP (SPATA19 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lung using 16656-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16656-1-AP (SPATA19 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung using 16656-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16656-1-AP (SPATA19 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary using 16656-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16656-1-AP (SPATA19 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 16656-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16656-1-AP (SPATA19 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 16656-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16656-1-AP (SPATA19 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 16656-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16656-1-AP (SPATA19 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of mouse testis using 16656-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using SPATA19 antibody (16656-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse testis using 16656-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using SPATA19 antibody (16656-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu testiculaire humain, tissu testiculaire de rat, tissu testiculaire de souris
• Résultats positifs en IHC: tissu testiculaire humain, tissu cutané humain, tissu hépatique humain, tissu ovarien humain, tissu placentaire humain, tissu pulmonaire humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu testiculaire de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

16656-1-AP cible SPATA19 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SPATA19 Protéine recombinante Ag10108
• Nom complet: spermatogenesis associated 19
• Masse moléculaire calculée: 167 aa, 19 kDa
• Poids moléculaire observé: 19 kDa
• Numéro d’acquisition GenBank: BC058039
• Symbole du gène: SPATA19
• Identification du gène (NCBI): 219938
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB,IF	Development; MFN2 interacts with nuage-associated proteins and is essential for male germ cell development by controlling mRNA fate during spermatogenesis; Authors -
mouse	WB,IF	Development; MFN2 interacts with nuage-associated proteins and is essential for male germ cell development by controlling mRNA fate during spermatogenesis.; Authors - Xiaoli Wang