Anticorps Polyclonal de lapin anti-SPATS2L

• Phare
• Validé par KD/KO

SPATS2L Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 16938-1-AP

Synonymes

DNAPTP6, LOC26010, SPATS2 like protein, SPATS2L

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Galerie de données de validation

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  WB analysis of HepG2 using 16938-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16938-1-AP (SPATS2L antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 16938-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16938-1-AP (SPATS2L antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 16938-1-AP

  Immunofluorescent analysis of HepG2 cells, using LOC26010 antibody 16938-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HepG2, tissu rénal de souris
• Résultats positifs en IHC: tissu rénal humain, tissu cardiaque humain, tissu cutané humain, tissu hépatique humain, tissu ovarien humain, tissu placentaire humain, tissu pulmonaire humain, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

16938-1-AP cible SPATS2L dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SPATS2L Protéine recombinante Ag10438
• Nom complet: viral DNA polymerase-transactivated protein 6
• Masse moléculaire calculée: 488 aa, 54 kDa
• Poids moléculaire observé: 54-62 kDa
• Numéro d’acquisition GenBank: BC018736
• Symbole du gène: LOC26010
• Identification du gène (NCBI): 26010
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SPATS2L encodes spermatogenesis associated and serine-rich 2-like protein, also named DNAPTP6 (DNA polymerase transactivated protein 6) in human (PMID:11230166). SPATS2L acts as an important regulator of β2-adrenergic receptor down-regulation (PubMed: 25562107). MiR-1269a down-regulates the expression of SPATS2L and LRP6, thereby inhibiting the proliferation of liver cancer cells (PMID: 28081866, PMID:35252180). SPATS2L has several isoforms by alternative splicing.

Publications

Species	Application	Title
human	WB	Front Cell Dev Biol; A Novel Signature Constructed by RNA-Binding Protein Coding Genes to Improve Overall Survival Prediction of Glioma Patients.; Authors - Zewei Tu
human	IHC,WB	Sci Rep; Characteristic DNA methylation profiles of chorionic villi in recurrent miscarriage.; Authors - Yosuke Matsumoto ; KD Validated
human	WB	J Transl Med; Spermatogenesis associated serine rich 2 like plays a prognostic factor and therapeutic target in acute myeloid leukemia by regulating the JAK2/STAT3/STAT5 axis; Authors - Fenglin Li ; KD Validated
mouse	IF	Sci Adv; RNA G-quadruplex organizes stress granule assembly through DNAPTP6 in neurons; Authors - Sefan Asamitsu