Anticorps Polyclonal de lapin anti-SPCS1

• Phare
• Validé par KD/KO

SPCS1 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(4)

Avis (1)

N° de cat : 11847-1-AP

Synonymes

SPC12, SPC1, SPase 12 kDa subunit, HSPC033

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Galerie de données de validation

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  WB analysis using 11847-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11847-1-AP (SPCS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 11847-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11847-1-AP (SPCS1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human skin using 11847-1-AP

  human skin tissue were subjected to SDS PAGE followed by western blot with 11847-1-AP (SPCS1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IF Staining of BxPC-3 using 11847-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed BxPC-3 cells using SPCS1 antibody (11847-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules PC-3, tissu cutané humain
• Résultats positifs en IF/ICC: cellules BxPC-3,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 4 publications below

Informations sur le produit

11847-1-AP cible SPCS1 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SPCS1 Protéine recombinante Ag2407
• Nom complet: signal peptidase complex subunit 1 homolog (S. cerevisiae)
• Masse moléculaire calculée: 102 aa, 12 kDa
• Poids moléculaire observé: 12 kDa
• Numéro d’acquisition GenBank: BC000884
• Symbole du gène: SPCS1
• Identification du gène (NCBI): 28972
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Nature; A CRISPR screen defines a signal peptide processing pathway required by flaviviruses.; Authors - Rong Zhang ; KO Validated
human	WB	PLoS Pathog; Signal Peptidase Complex Subunit 1 Participates in the Assembly of Hepatitis C Virus through an Interaction with E2 and NS2.; Authors - Suzuki Ryosuke R ; KD Validated
	WB	PLoS Pathog; SPCS1-Dependent E2-p7 processing determines HCV Assembly efficiency.; Authors - Nabeel Alzahrani
human	WB	Mol Cell; Cleavage of the pseudoprotease iRhom2 by the signal peptidase complex reveals an ER-to-nucleus signaling pathway; Authors - Iqbal Dulloo

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Joao (Verified Customer) (10-09-2019)

Specific, single band (~12kDa), low background

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: 293T

SPCS1 Antibody Western Blot, validation (1:500 dilution) in 293T (Cat no:11847-1-AP)

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