Anticorps Polyclonal de lapin anti-SPHK2

• Phare
• Validé par KD/KO

SPHK2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

35

Avis (6)

N° de cat : 17096-1-AP

Synonymes

SK 2, sphingosine kinase 2, SPHK2, SPK 2

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Galerie de données de validation

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  WB analysis using 17096-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17096-1-AP (SPHK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17096-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17096-1-AP (SPHK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 17096-1-AP

  WB result of SPHK2 antibody (17096-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-SPHK2 transfected A549 cells.

• 

  WB analysis using 17096-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17096-1-AP (SPHK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17096-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17096-1-AP (SPHK2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 17096-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 17096-1-AP (SPHK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 17096-1-AP

  IP Result of anti-SPHK2 (IP:17096-1-AP, 4ug; Detection:17096-1-AP 1:800) with mouse liver tissue lysate 4000ug.

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  IHC staining of human breast cancer using 17096-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17096-1-AP (SPHK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 17096-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17096-1-AP (SPHK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 17096-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17096-1-AP (SPHK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 17096-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17096-1-AP (SPHK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 17096-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17096-1-AP (SPHK2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 17096-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17096-1-AP (SPHK2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HEK-293 using 17096-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 17096-1-AP (SPHK2 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 17096-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SPHK2 antibody (17096-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of HeLa using 17096-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SPHK2 (17096-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules L02, cellules A549, cellules HCT 116, cellules Raji, tissu cardiaque de souris, tissu cérébral de rat, tissu cérébral de souris, tissu hépatique de souris, tissu rénal de souris, tissu rénal humain
• Résultats positifs en IP: tissu hépatique de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu hépatique humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 9 publications below
• WB: See 31 publications below
• IHC: See 5 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• FC: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

17096-1-AP cible SPHK2 dans les applications de WB, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SPHK2 Protéine recombinante Ag10569
• Nom complet: sphingosine kinase 2
• Masse moléculaire calculée: 618 aa, 65 kDa
• Poids moléculaire observé: 65-70 kDa
• Numéro d’acquisition GenBank: BC010671
• Symbole du gène: SPHK2
• Identification du gène (NCBI): 56848
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SPHK2(Sphingosine kinase 2) is also named as SK2, SPK2 and catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. SPHK2 associates with HDAC1 and HDAC2 in repressor complexes and is selectively enriched at the promoters of the genes encoding the cyclin-dependent kinase inhibitor p21 or the transcriptional regulator c-fos, where it enhanced local histone H3 acetylation and transcription.SphK2 is a ~66kDa protein that is most abundantly expressed in the liver and heart(PMID:19168577). SphK2 has two splice variants, which are the 68-kDa SphK2-S and the NH2-terminal extended 72-kDa SphK2-L (PMID:17974990).

Publications

Species	Application	Title
human	WB	Circ Res; Altered Smooth Muscle Cell Histone Acetylome by the SPHK2/S1P Axis Promotes Pulmonary Hypertension; Authors - A Dushani C U Ranasinghe
human	WB	Blood; Targeting sphingosine kinase 1 induces MCL1 dependent cell death in acute myeloid leukemia.; Authors - Jason A Powell
human,rat	WB,IHC,IF	Bioact Mater; Injectable cartilage matrix hydrogel loaded with cartilage endplate stem cells engineered to release exosomes for non-invasive treatment of intervertebral disc degeneration.; Authors - Liwen Luo
human	WB,IF,IP	Oncogene; Cytoplasmic dynein regulates the subcellular localization of sphingosine kinase 2 to elicit tumor-suppressive functions in glioblastoma.; Authors - Heidi A Neubauer
human	WB	PLoS Pathog; Sphingosine kinase 1/S1P receptor signaling axis is essential for cellular uptake of Neisseria meningitidis in brain endothelial cells; Authors - Ingo Fohmann
human	CoIP	PLoS Pathog; Enzymatic independent role of sphingosine kinase 2 in regulating the expression of type I interferon during influenza A virus infection; Authors - Mengqiong Xu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Yasuyo (Verified Customer) (02-23-2023)

Clear staining in mouse kidney paraffin section

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1 to 200
• Cell Tissue Type: kidney

SPHK2 Antibody Immunohistochemistry validation (1 to 200 dilution) in kidney (Cat no:17096-1-AP)

FH

Hua (Verified Customer) (01-23-2023)

Good antibody. Works well on WB.

• Applications: Western Blot
• Primary Antibody Dilution: 1:4000
• Cell Tissue Type: mouse liver and mouse brain

SPHK2 Antibody Western Blot validation (1:4000 dilution) in mouse liver and mouse brain (Cat no:17096-1-AP)

FH

Jason (Verified Customer) (06-09-2021)

Clear staining in mouse liver paraffin section.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: liver

SPHK2 Antibody Immunohistochemistry validation (1:100 dilution) in liver (Cat no:17096-1-AP)

FH

David (Verified Customer) (05-04-2021)

It works for cell IF in vitro.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: A549 cell line

SPHK2 Antibody Immunofluorescence validation (1:100 dilution) in A549 cell line (Cat no:17096-1-AP)

FH

Chen (Verified Customer) (03-13-2021)

Works good for cell immunofluorescence assay in vitro.

• Applications: Immunofluorescence
• Cell Tissue Type: Heart cells

SPHK2 Antibody Immunofluorescence validation ( dilution) in Heart cells (Cat no:17096-1-AP)

FH

Wei (Verified Customer) (03-12-2020)

Sharp and clear band from mouse heart lysates

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart