Anticorps Polyclonal de lapin anti-SPI1

• Phare
• Validé par KD/KO

SPI1 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications(7)

Avis (1)

N° de cat : 55100-1-AP

Synonymes

31 kDa transforming protein, OF, PU.1, SFPI1, SPI 1, SPI A, SPI1, Transcription factor PU.1

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Galerie de données de validation

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  WB analysis using 55100-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55100-1-AP (SPI1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW264.7 using 55100-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 55100-1-AP (SPI1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HepG2, RAW264.7, tissu hépatique de rat, tissu hépatique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IF: See 1 publications below

Informations sur le produit

55100-1-AP cible SPI1 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: spleen focus forming virus (SFFV) proviral integration oncogene spi1
• Masse moléculaire calculée: 31 kDa
• Poids moléculaire observé: 31-42 kDa
• Numéro d’acquisition GenBank: NM_003120
• Symbole du gène: SPI1
• Identification du gène (NCBI): 6688
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SPI1 binds to the PU-box which is a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. SPI1 is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. It also binds RNA and may modulate pre-mRNA splicing. The antibody is specific to SPI1. SPI1 can be detected 37-42 kDa (PMID: 28362429, 31586032, 28586009).

Publications

Species	Application	Title
	IF	Mucosal Immunol; IL-33/ST2/IL-9/IL-9R signaling disrupts ocular surface barrier in allergic inflammation.; Authors - Jiaoyue Hu
mouse	WB	Sci Rep; Specific immunotherapy in combination with Clostridium butyricum inhibits allergic inflammation in the mouse intestine.; Authors - Yanhong Shi
human	WB	J Leukoc Biol; miR-199a-5p inhibits monocyte/macrophage differentiation by targeting the activin A type 1B receptor gene and finally reducing C/EBPα expression.; Authors - Hai-Shuang Lin ; KD Validated
human	WB	Med Oncol; PU.1 negatively regulates tumorigenesis in non-small-cell lung cancer; Authors - Xiaoxiao Liu
human	WB	Exp Cell Res; An innovative immunotherapeutic strategy for rheumatoid arthritis: Selectively suppressing angiogenesis and osteoclast differentiation by fully human antibody targeting thymocyte antigen-1; Authors - Xuanxuan Hu
human	WB	Cell Rep; A CLIC1 network coordinates matrix stiffness and the Warburg effect to promote tumor growth in pancreatic cancer; Authors - Jia-Hao Zheng

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Andrew (Verified Customer) (08-09-2021)

When used in our capillary electrophoresis equipment (JESS), the proteintech antibody for SPI1 seemed to show specific binding to a protein at around 60KD. We believe this may be recognising a dimerized form of the protein. This detection was consistent between lanes and responded as expected to varying amounts of sample protein and antibody concentration.

• Applications: Other
• Primary Antibody Dilution: Various
• Cell Tissue Type: Human brain tissue

SPI1 Antibody Other validation (Various dilution) in Human brain tissue (Cat no:55100-1-AP)

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