Anticorps Polyclonal de lapin anti-SPIN1

• Phare
• Validé par KD/KO

SPIN1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ChIP, ELISA

Conjugaison

Non conjugué

Publications(14)

N° de cat : 12105-1-AP

Synonymes

Spindlin1, spindlin 1, SPIN 1, SPIN, Ovarian cancer-related protein

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Galerie de données de validation

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  WB analysis using 12105-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12105-1-AP (SPIN1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12105-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12105-1-AP (SPIN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12105-1-AP

  WB result of SPIN1 antibody (12105-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SPIN1 transfected HeLa cells.

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  IP experiment of HeLa using 12105-1-AP

  IP result of anti-SPIN1 (IP:12105-1-AP, 3ug; Detection:12105-1-AP 1:800) with HeLa cells lysate 1840ug.

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  IF Staining of HEK-293 using 12105-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using SPIN1 antibody (12105-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules PC-3
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IF/ICC: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 8 publications below
• WB: See 14 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 2 publications below
• ChIP: See 1 publications below

Informations sur le produit

12105-1-AP cible SPIN1 dans les applications de WB, IHC, IF/ICC, IP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SPIN1 Protéine recombinante Ag2750
• Nom complet: spindlin 1
• Masse moléculaire calculée: 262 aa, 30 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC013571
• Symbole du gène: SPIN1
• Identification du gène (NCBI): 10927
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SPIN1 (Spindlin 1) is a histone methylation reader, which recognizes trimethylated histone H3 lysine 4 through the protein-protein interaction. SPIN1 was initially found in ovarian cancer via searching ovarian cancer-related genes . It is overexpressed in ovarian cancer tissue, which exhibits a high homology to mouse Spin (Spindlin) gene. Signaling pathways responsible for SPIN1 functions include PI3K/Akt, Wnt and RET that are highly relevant to tumorigenesis.(PMID: 34492335, PMID: 31790762)

Publications

Species	Application	Title
human	WB	Nat Commun; Structural mechanism of bivalent histone H3K4me3K9me3 recognition by the Spindlin1/C11orf84 complex in rRNA transcription activation.; Authors - Yongming Du ; KD Validated
human	WB	Elife; SPIN1 promotes tumorigenesis by blocking the uL18 (universal large ribosomal subunit protein 18)-MDM2-p53 pathway in human cancer.; Authors - Ziling Fang ; KD Validated
human	WB, IP	PLoS Pathog; The Tudor Domain Protein Spindlin1 Is Involved in Intrinsic Antiviral Defense against Incoming Hepatitis B Virus and Herpes Simplex Virus Type 1.; Authors - Aurélie Ducroux ; KD Validated
human	WB,IF	EMBO Rep; Nucleolar protein Spindlin1 recognizes H3K4 methylation and stimulates the expression of rRNA genes.; Authors - Wang Weixiang W ; KD Validated
human	WB,IHC	Mol Oncol; E2F1-activated SPIN1 promotes tumor growth via a MDM2-p21-E2F1 feedback loop in gastric cancer.; Authors - Bei-Bei Lv ; KD Validated
human	WB	ACS Biomater Sci Eng; Soft Substrate Promotes Osteosarcoma Cell Self-Renewal, Differentiation, and Drug Resistance Through miR-29b and Its Target Protein Spin 1.; Authors - Shun Li

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