Anticorps Polyclonal de lapin anti-SPIN1-Specific

• Phare
• Validé par KD/KO

SPIN1-Specific Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 19531-1-AP

Synonymes

OCR, Ovarian cancer related protein, SPIN, SPIN1, SPIN1-Specific, spindlin 1

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Galerie de données de validation

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  WB analysis of SKOV-3 using 19531-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 19531-1-AP (SPIN1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A2780 using 19531-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 19531-1-AP (SPIN1-Specific antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 19531-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19531-1-AP (SPIN1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 19531-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 19531-1-AP (SPIN1-Specific antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary tumor using 19531-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 19531-1-AP (SPIN1-Specific antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 19531-1-AP

  Immunofluorescent analysis of Hela cells, using SPIN1 antibody 19531-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules SKOV-3, cellules A2780, cellules HEK-293
• Résultats positifs en IHC: tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 2 publications below

Informations sur le produit

19531-1-AP cible SPIN1-Specific dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: spindlin 1
• Masse moléculaire calculée: 30 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: NM_006717
• Symbole du gène: SPIN1
• Identification du gène (NCBI): 10927
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SPIN1 (Spindlin 1) is a histone methylation reader, which recognizes trimethylated histone H3 lysine 4 through the protein-protein interaction. SPIN1 was initially found in ovarian cancer via searching ovarian cancer-related genes . It is overexpressed in ovarian cancer tissue, which exhibits a high homology to mouse Spin (Spindlin) gene. Signaling pathways responsible for SPIN1 functions include PI3K/Akt, Wnt and RET that are highly relevant to tumorigenesis.(PMID: 34492335, PMID: 31790762)

Publications

Species	Application	Title
human	WB	Oncotarget; Suppressive role exerted by microRNA-29b-1-5p in triple negative breast cancer through SPIN1 regulation.; Authors - Rosa Drago-Ferrante ; KD Validated
human	WB	J Cell Biochem; Molecular basis underlying the biological effects elicited by extremely low-frequency magnetic field (ELF-MF) on neuroblastoma cells.; Authors - Sulpizio Marilisa M
human	IHC	Bioengineered; The microRNA-381(miR-381)/Spindlin1(SPIN1) axis contributes to cell proliferation and invasion of colorectal cancer cells by regulating the Wnt/β-catenin pathway.; Authors - Ling Zhou
human	IHC	J Exp Clin Cancer Res; SPIN1, negatively regulated by miR-148/152, enhances Adriamycin resistance via upregulating drug metabolizing enzymes and transporter in breast cancer.; Authors - Xu Chen