Anticorps Polyclonal de lapin anti-SPON2

• Phare
• Validé par KD/KO

SPON2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 20513-1-AP

Synonymes

DIL 1, DIL1, M spondin, Mindin, SPON2, Spondin 2

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Galerie de données de validation

• 

  WB analysis of LNCaP using 20513-1-AP

  LNCaP cells were subjected to SDS PAGE followed by western blot with 20513-1-AP (SPON2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 20513-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 20513-1-AP (SPON2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 20513-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 20513-1-AP (SPON2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate hyperplasia using 20513-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 20513-1-AP (SPON2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate hyperplasia using 20513-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 20513-1-AP (SPON2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 20513-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 20513-1-AP (SPON2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 20513-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 20513-1-AP (SPON2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 20513-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 20513-1-AP (SPON2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 20513-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 20513-1-AP (SPON2 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules LNCaP,
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu d'hyperplasie de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Informations sur le produit

20513-1-AP cible SPON2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SPON2 Protéine recombinante Ag14337
• Nom complet: spondin 2, extracellular matrix protein
• Masse moléculaire calculée: 331 aa, 36 kDa
• Poids moléculaire observé: 38 kDa
• Numéro d’acquisition GenBank: BC002707
• Symbole du gène: SPON2
• Identification du gène (NCBI): 10417
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SPON2 (Spondin 2), also known as Mindin, is a member of the F-spondin family of secreted extracellular matrix proteins. This protein has been implicated in axon guidance, immune response and adhesion. SPON2 is essential in the initiation of the innate immune response and represents a unique pattern-recognition molecule in the ECM for microbial pathogens (PMID: 14691481). It also functions as an integrin ligand for inflammatory cell recruitment and T-cell priming (PMID: 19153605). Besides, SPON2 has been reported as a prognostic biomarker for ovarian and prostate cancer (PMID: 17490732; 22615945; 23665271).

Publications

Species	Application	Title
human	IF	Nature; Spatial immune scoring system predicts hepatocellular carcinoma recurrence; Authors - Gengjie Jia
human	WB	J Cell Mol Med; Spondin 2 promotes the proliferation, migration and invasion of gastric cancer cells.; Authors - Haoming Lu ; KD Validated
human,mouse	WB,IF	Front Cell Dev Biol; Transcriptional Activation of Matricellular Protein Spondin2 (SPON2) by BRG1 in Vascular Endothelial Cells Promotes Macrophage Chemotaxis.; Authors - Nan Li
human	IHC	Front Cell Dev Biol; Integrative Analysis of DNA Methylation and Transcriptome Identifies a Predictive Epigenetic Signature Associated With Immune Infiltration in Gliomas.; Authors - Jianlei Zhang
human	IHC	Front Oncol; Knockdown of SPON2 inhibits the growth of triple-negative breast cancer; Authors - Xueyi Hu
human	IHC	Cancer Res; Combining Spatial Transcriptomics, Pseudotime, and Machine Learning Enables Discovery of Biomarkers for Prostate Cancer; Authors - Martin Smelik

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