Anticorps Polyclonal de lapin anti-SPPL2A

SPPL2A Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC,ELISA

Conjugaison

Non conjugué

N° de cat : 13255-1-AP

Synonymes

IMP 3, IMP3, Intramembrane protease 3, Presenilin like protein 2, PSL2, SPP like 2A, SPPL2A

---

Galerie de données de validation

• 

  WB analysis of human colon using 13255-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 13255-1-AP (SPPL2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human colon using 13255-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 13255-1-AP (SPPL2A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 13255-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13255-1-AP (SPPL2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 13255-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 13255-1-AP (SPPL2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 13255-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13255-1-AP (SPPL2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 13255-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13255-1-AP (SPPL2A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 13255-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13255-1-AP (SPPL2A antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 13255-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 13255-1-AP (SPPL2A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 13255-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 13255-1-AP (SPPL2A antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 13255-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13255-1-AP (SPPL2A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 13255-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13255-1-AP (SPPL2A antibody) at dilution of 1:50 (under 40x lens).

---

Applications testées

• Résultats positifs en WB: tissu de côlon humain, cellules Jurkat, tissu de côlon de souris
• Résultats positifs en IHC: tissu testiculaire humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

13255-1-AP cible SPPL2A dans les applications de WB, IHC,ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SPPL2A Protéine recombinante Ag3935
• Nom complet: signal peptide peptidase-like 2A
• Masse moléculaire calculée: 520 aa, 58 kDa
• Poids moléculaire observé: 58 kDa
• Numéro d’acquisition GenBank: BC025740
• Symbole du gène: SPPL2A
• Identification du gène (NCBI): 84888
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SPPL2A(Signal peptide peptidase-like 2A) is also named as IMP3(Intramembrane protease 3), PSL2(Presenilin-like protein 2) and belongs to the peptidase A22B family. It is an aspartyl intramembrane protease, has been implicated in the proteolysis of TNF-alpha, Fas Ligand and Bri2 and endogenous signal-peptide-peptidase-like2A (SPPL2a) is localised in lysosomes/late endosomes(PMID:21896273). This protein has a signal peptide with 25 amino acid and seven glycosylation sites.