Anticorps Polyclonal de lapin anti-SPRR3

• Phare
• Validé par KD/KO

SPRR3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(11)

N° de cat : 11742-1-AP

Synonymes

SPRC, Small proline-rich protein 3

---

Galerie de données de validation

• 

  WB analysis of HeLa using 11742-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11742-1-AP (SPRR3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 11742-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11742-1-AP (SPRR3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of COLO 320 using 11742-1-AP

  IP result of anti-SPRR3 (IP:11742-1-AP, 3ug; Detection:11742-1-AP 1:500) with COLO 320 cells lysate 1720ug.

• 

  IHC staining of human colon cancer using 11742-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11742-1-AP (SPRR3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human colon cancer using 11742-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11742-1-AP (SPRR3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human breast cancer using 11742-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11742-1-AP (SPRR3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human breast cancer using 11742-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11742-1-AP (SPRR3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 11742-1-AP

  Immunofluorescent analysis of HeLa cells using 11742-1-AP (SPRR3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules HeLa, tissu hépatique humain
• Résultats positifs en IP: cellules COLO 320
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 10 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

11742-1-AP cible SPRR3 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SPRR3 Protéine recombinante Ag2337
• Nom complet: small proline-rich protein 3
• Masse moléculaire calculée: 19 kDa
• Poids moléculaire observé: 22-25 kDa
• Numéro d’acquisition GenBank: BC017802
• Symbole du gène: SPRR3
• Identification du gène (NCBI): 6707
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SPRR3, also named as Esophagin, Cornifin beta and SPRC, belongs to the cornifin (SPRR) family. It is cross-linked envelope protein of keratinocytes. Increased expression of SPRR3 appears to be involved in colorectal tumorigenesis. SPRR3 is a marker for terminal squamous cell differentiation.a potential novel therapeutic target for less advanced stages of Breast Cancer.

Publications

Species	Application	Title
human,mouse,rat	WB,IP	FASEB J; Small proline-rich repeat 3 is a novel coordinator of PDGFRβ and integrin β1 crosstalk to augment proliferation and matrix synthesis by cardiac fibroblasts.; Authors - Sarika Saraswati ; KO Validated
human	WB,IHC	Mol Med; Upregulation of SPRR3 promotes colorectal tumorigenesis.; Authors - Cho Dong-Hyung DH ; KD Validated
human	WB,IHC	Breast Cancer Res Treat; Expression of SPRR3 is associated with tumor cell proliferation in less advanced stages of breast cancer.; Authors - Kim Jin Cheon JC
human	WB	Arch Biochem Biophys; Investigation of mouse amniotic fluid for stimulating ability of keratinocyte differentiation depending on the fetal stage.; Authors - Miki Kuribayashi
human	WB	Onco Targets Ther; Dysregulation of SPRR3/miR-876-3p Axis Contributes to Tumorigenesis in Non-Small-Cell Lung Cancer.; Authors - Qin Li
human	WB	Med; A tumor cornification and immune-infiltration-based scheme for anti-PD-1 plus chemotherapy response in advanced squamous cell lung carcinoma; Authors - Minlin Jiang

{{ptg:RelatedPrimaryAntibodies}}