Anticorps Polyclonal de lapin anti-SPRY2

• Phare
• Validé par KD/KO

SPRY2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(8)

Avis (2)

N° de cat : 11383-1-AP

Synonymes

Spry-2, Spry 2, sprouty homolog 2 (Drosophila), Protein sprouty homolog 2, hSPRY2

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Galerie de données de validation

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  WB analysis using 11383-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11383-1-AP (SPRY2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of SW 1990 using 11383-1-AP

  SW 1990 cells were subjected to SDS PAGE followed by western blot with 11383-1-AP (SPRY2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 11383-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 11383-1-AP (SPRY2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 11383-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11383-1-AP (SPRY2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas cancer using 11383-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11383-1-AP (SPRY2 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of A375 using 11383-1-AP

  Immunofluorescent analysis of A375 cells using 11383-1-AP (SPRY2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules SW-1990, cellules A375, cellules Jurkat, cellules Neuro-2a
• Résultats positifs en IHC: tissu de cancer du pancréas humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A375

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

11383-1-AP cible SPRY2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SPRY2 Protéine recombinante Ag1918
• Nom complet: sprouty homolog 2 (Drosophila)
• Masse moléculaire calculée: 35 kDa
• Poids moléculaire observé: 35-40 kDa, 60~65 kDa
• Numéro d’acquisition GenBank: BC015745
• Symbole du gène: SPRY2
• Identification du gène (NCBI): 10253
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Sprouty(SPRY) genes encode intracellular inhibitors of receptor tyrosine kinase (RTK) signaling pathways, including those triggered by fibroblast growth factors (Fgfs). In addition to its well-reported function as an inhibitor of the RAS/RAF/MEK signalling cascade, SPRY2 exerts its inhibitory role in the PI3K/AKT signalling pathway through PTEN, and inhibits ERK/MAPK activity via EGFR trafficking. SPRY2 was detected 60-65 kDa (PMID:29291435).

Publications

Species	Application	Title
mouse	WB,IF	J Crohns Colitis; MSCs-exosomes can promote macrophage M2 polarization via exosomal miR-21-5p through Mesenteric injection: a promising way to attenuate murine colitis; Authors - Wenwei Qian
mouse	WB	Int J Mol Sci; Kub3 Deficiency Causes Aberrant Late Embryonic Lung Development in Mice by the FGF Signaling Pathway.; Authors - Guangying Yang
human	WB	J Cell Mol Med; Exosomal miR-27 negatively regulates ROS production and promotes granulosa cells apoptosis by targeting SPRY2 in OHSS.; Authors - Kailu Liu ; KD Validated
human	WB	Toxicol Appl Pharmacol; Hexachlorophene, a selective SHP2 inhibitor, suppresses proliferation and metastasis of KRAS-mutant NSCLC cells by inhibiting RAS/MEK/ERK and PI3K/AKT signaling pathways.; Authors - Nai-Jie Fu
mouse	WB	Cell Biol Int; Sprouty2 is involved in the control of osteoblast proliferation and differentiation through the FGF and BMP signaling pathways.; Authors - Takaharu Taketomi
mouse	IHC	Front Immunol; Activated hepatic stellate cell-derived small extracellular vesicles facilitate M2 macrophage polarization and hepatoma progression via miR-27a-3p; Authors - Yufeng Sun

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Christine (Verified Customer) (01-27-2023)

Detects two bands on HEK293T lysates. main one between 36 and 48 kDa, second one between 48 and 85 kDa, in accordance with datasheet

• Applications: Western Blot
• Primary Antibody Dilution: 1:1,000
• Cell Tissue Type: HEK293T

FH

Silvia (Verified Customer) (01-31-2022)

The antibody worked really well and gave me a clear band on Western Blot around 50 kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:800
• Cell Tissue Type: Human adherent cancer cells

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