Anticorps Polyclonal de lapin anti-SPTBN1-Specific

SPTBN1-Specific Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

Avis (1)

N° de cat : 19722-1-AP

Synonymes

Beta II spectrin, beta-fodrin, betaSpII, ELF, Fodrin beta chain, Spectrin beta chain, brain 1, SPTB2, SPTBN1, SPTBN1-Specific

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Galerie de données de validation

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  WB analysis of mouse brain using 19722-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19722-1-AP (SPTBN1-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 19722-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 19722-1-AP (SPTBN1-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 19722-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 19722-1-AP (SPTBN1-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 19722-1-AP

  IP Result of anti-SPTBN1-Specific (IP:19722-1-AP, 5ug; Detection:19722-1-AP 1:600) with HEK-293 cells lysate 1000ug.

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  IHC staining of human liver cancer using 19722-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19722-1-AP (SPTBN1-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 19722-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 19722-1-AP (SPTBN1-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 19722-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19722-1-AP (SPTBN1-Specific antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 19722-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19722-1-AP (SPTBN1-Specific antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 19722-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 19722-1-AP (SPTBN1-Specific antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu pulmonaire de souris, tissu rénal de souris
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer du pancréas humain, tissu pulmonaire humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

19722-1-AP cible SPTBN1-Specific dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: spectrin, beta, non-erythrocytic 1
• Masse moléculaire calculée: 275 kDa
• Poids moléculaire observé: 275 kDa
• Numéro d’acquisition GenBank: NM_003128
• Symbole du gène: SPTBN1
• Identification du gène (NCBI): 6711
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SPTBN1 is the non-erythrocytic form of β-spectrin or β-fodrin. Spectrins are members of the superfamily of F-actin cross linking proteins that are important as scaffolding proteins for protein sorting, cell adhesion, and migration. Reduced expression of SPTBN1 has been found to correlate with shorter survival of patients with hepatocellular cancer, pancreatic cancer and other malignancies of the gastrointestinal tract, suggesting tumor suppression ability of this protein.

Publications

Species	Application	Title
human	WB,IP	Nat Commun; USP9X regulates centrosome duplication and promotes breast carcinogenesis.; Authors - Xin Li
human	WB	Anal Chem; Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.; Authors - Hongwei Chu
human	WB,IHC	Proteomics; Global profiling of proteolytically modified proteins in human metastatic hepatocellular carcinoma cell lines reveals CAPN2 centered network.; Authors - Shen Chengpin C
human	IF	Cancer Med; MiR-362 suppresses cervical cancer progression via directly targeting BAP31 and activating TGFβ/Smad pathway.; Authors - Shuya Yang
mouse	IF	FEBS Open Bio; Identification of nuclear phosphoproteins as novel tobacco markers in mouse lung tissue following short-term exposure to tobacco smoke.; Authors - Kanako Niimori-Kita
mouse,human	WB	Neuron; Species-specific FMRP regulation of RACK1 is critical for prenatal cortical development; Authors - Minjie Shen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Ruchi (Verified Customer) (01-25-2023)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Mouse skin