Anticorps Polyclonal de lapin anti-SPTLC1

• Phare
• Validé par KD/KO

SPTLC1 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

13

N° de cat : 15376-1-AP

Synonymes

HSAN, HSAN1, HSN1, LBC1, LCB 1, LCB1, Serine palmitoyltransferase 1, SPT 1, SPT1, SPTI, SPTLC1

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Galerie de données de validation

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  WB analysis of Jurkat using 15376-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15376-1-AP (SPTLC1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 15376-1-AP

  WB result of SPTLC1 antibody (15376-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SPTLC1 transfected HEK-293 cells.

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  WB analysis of HepG2 using 15376-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15376-1-AP (SPTLC1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 15376-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15376-1-AP (SPTLC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15376-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15376-1-AP (SPTLC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 15376-1-AP

  IP Result of anti-SPTLC1 (IP:15376-1-AP, 4ug; Detection:15376-1-AP 1:500) with HEK-293 cells lysate 880ug.

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  IHC staining of human colon cancer using 15376-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15376-1-AP (SPTLC1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon cancer using 15376-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15376-1-AP (SPTLC1 antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HEK-293, cellules HepG2, tissu hépatique humain
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IHC: See 2 publications below

Informations sur le produit

15376-1-AP cible SPTLC1 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SPTLC1 Protéine recombinante Ag1162
• Nom complet: serine palmitoyltransferase, long chain base subunit 1
• Masse moléculaire calculée: 53 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC007085
• Symbole du gène: SPTLC1
• Identification du gène (NCBI): 10558
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SPTLC1 is a subunit of serine palmitoyltransferase (SPT) which is the key enzyme in sphingolipid biosynthesis and is essential for embryogenesis and cell survival. Mutations in the SPTLC1 gene (C133W, C133Y, V144D, and G387A) were reported to be responsible for the development of an inherited sensory neuropathy (hereditary sensory neuropathy type I, HSN1).

Publications

Species	Application	Title
rat	IHC	Cell Metab; Central Ceramide Signaling Mediates Obesity-Induced Precocious Puberty.; Authors - Violeta Heras
human	WB	Sci Adv; Inhibition of sphingolipid de novo synthesis counteracts muscular dystrophy.; Authors - Pirkka-Pekka Laurila
mouse	WB	J Neurosci; MicroRNA-137/181c regulates serine palmitoyltransferase and in turn amyloid β, novel targets in sporadic Alzheimer's disease.; Authors - Hirosha Geekiyanage
mouse	WB,IHC	Neurobiol Aging; Inhibition of serine palmitoyltransferase reduces Aβ and tau hyperphosphorylation in a murine model: a safe therapeutic strategy for Alzheimer's disease.; Authors - Hirosha Geekiyanage
mouse	WB	Am J Physiol Lung Cell Mol Physiol; Neutrophil elastase increases airway ceramide levels via upregulation of serine palmitoyltransferase.; Authors - Sophia Karandashova
human	WB	Front Cell Neurosci; Activation of Mitochondrial Unfolded Protein Response in SHSY5Y Expressing APP Cells and APP/PS1 Mice.; Authors - Yang Shen ; KD Validated