Anticorps Polyclonal de lapin anti-SQRDL

• Phare
• Validé par KD/KO

SQRDL Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

22

N° de cat : 17256-1-AP

Synonymes

CGI 44, SQOR, SQRDL

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Galerie de données de validation

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  WB analysis using 17256-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17256-1-AP (SQRDL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 17256-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17256-1-AP (SQRDL antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17256-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17256-1-AP (SQRDL antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17256-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17256-1-AP (SQRDL antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 17256-1-AP

  IP Result of anti-SQRDL (IP:17256-1-AP, 4ug; Detection:17256-1-AP 1:500) with HeLa cells lysate 2000ug.

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  IHC staining of human brain using 17256-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17256-1-AP (SQRDL antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 17256-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17256-1-AP (SQRDL antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human colon cancer using 17256-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17256-1-AP (SQRDL antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 17256-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17256-1-AP (SQRDL antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 17256-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 17256-1-AP (SQRDL antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 17256-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 17256-1-AP (SQRDL antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human spleen using 17256-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17256-1-AP (SQRDL antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 17256-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17256-1-AP (SQRDL antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 17256-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17256-1-AP (SQRDL antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 17256-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17256-1-AP (SQRDL antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 17256-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17256-1-AP (SQRDL antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung using 17256-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17256-1-AP (SQRDL antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG, cellules HepG2, cellules HT-29, cellules THP-1
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu cérébral humain, tissu de cancer du côlon humain, tissu de cancer du foie humain, tissu hépatique de rat, tissu hépatique de souris, tissu pulmonaire humain, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 18 publications below
• IHC: See 4 publications below

Informations sur le produit

17256-1-AP cible SQRDL dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SQRDL Protéine recombinante Ag10762
• Nom complet: sulfide quinone reductase-like (yeast)
• Masse moléculaire calculée: 450 aa, 50 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC016836
• Symbole du gène: SQRDL
• Identification du gène (NCBI): 58472
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SQRDL(Sulfide:quinone oxidoreductase, mitochondrial) catalyzes the oxidation of hydrogen sulfide, with the help of a quinone.Although the SQRDL precursor protein displays a molecular weight of 50 kDa,a prominent band at about 46 kDa is detected by western blot. This discrepancy reflects the cleavage of an N-terminal targeting sequence of 4 kDa during the import of the protein into the mitochondria(PMID:22067608).SQRDL is one of the enzymes involved in H2S signaling in a discrete population of neurons, oligodendrocytes, and endothelial cells.

Publications

Species	Application	Title
mouse	WB	Cell; Endogenous Hydrogen Sulfide Production Is Essential for Dietary Restriction Benefits.; Authors - Christopher Hine ; KD Validated
mouse,rat	WB	Nat Commun; Sulfide catabolism ameliorates hypoxic brain injury.; Authors - Eizo Marutani ; KD Validated
mouse	WB	EMBO Mol Med; β-RA reduces DMQ/CoQ ratio and rescues the encephalopathic phenotype in Coq9 R239X mice.; Authors - Agustín Hidalgo-Gutiérrez
human	WB	EMBO Mol Med; Coenzyme Q deficiency causes impairment of the sulfide oxidation pathway.; Authors - Marcello Ziosi
mouse	WB	EMBO Mol Med; CoQ deficiency causes disruption of mitochondrial sulfide oxidation, a new pathomechanism associated with this syndrome.; Authors - Marta Luna-Sánchez
mouse	WB	Antioxid Redox Signal; The Cysteine Dioxgenase Knockout Mouse: Altered Cysteine Metabolism in Nonhepatic Tissues Leads to Excess H2S/HS(-) Production and Evidence of Pancreatic and Lung Toxicity.; Authors - Roman Heather B HB