Anticorps Polyclonal de lapin anti-SRA1

SRA1 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 24655-1-AP

Synonymes

pp7684, SRA, SRA1, SRAP, STRAA1

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Galerie de données de validation

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  WB analysis of A2780 using 24655-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 24655-1-AP (SRA1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 24655-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24655-1-AP (SRA1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A2780 using 24655-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 24655-1-AP (SRA1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 24655-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24655-1-AP (SRA1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 24655-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24655-1-AP (SRA1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse testis using 24655-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24655-1-AP (SRA1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 24655-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24655-1-AP (SRA1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 24655-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed MCF-7 cells using 24655-1-AP (SRA1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of MCF-7 using 24655-1-AP

  1X10^6 MCF-7 cells were stained with 0.2ug SRA1 antibody (24655-1-AP, red) and control antibody (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: cellules A2780, cellules HEK-293
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MCF-7
• Résultats positifs en cytométrie: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IHC: See 1 publications below

Informations sur le produit

24655-1-AP cible SRA1 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SRA1 Protéine recombinante Ag20082
• Nom complet: steroid receptor RNA activator 1
• Masse moléculaire calculée: 26 kDa
• Poids moléculaire observé: 30 kDa, 33 kDa
• Numéro d’acquisition GenBank: BC067895
• Symbole du gène: SRA1
• Identification du gène (NCBI): 10011
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Steroid receptor RNA activator 1 (SRA1), also known as seroid receptor RNA activator protein (SRAP) or SRA, is involved in modulating the activity of multiple transcription factors including the estrogen receptor (ER) (PMID: 20398657). It may play a role in tumorigenesis (PMID: 16152589). The SRA1 gene encodes both SRA1 protein and a non-coding functional RNA that functions as part of a ribonucleoprotein complex activating steroid receptor induced transcription. This polyclonal antibody recognizes endogenous SRA1 isoforms which migrate as a doublet on SDS-PAGE gels (PMID: 12565891; 23907597).

Publications

Species	Application	Title
mouse	WB	Free Radic Biol Med; Up-regulation of thioredoxin system by puerarin inhibits lipid uptake in macrophages; Authors - Wenchao Li,
human, mouse	WB	Arterioscler Thromb Vasc Biol; RP5-833A20.1/miR-382-5p/NFIA-Dependent Signal Transduction Pathway Contributes to the Regulation of Cholesterol Homeostasis and Inflammatory Reaction.; Authors - Yan-Wei Hu
human	WB	J Lipid Res; A lincRNA-DYNLRB2-2/GPR119/GLP-1R/ABCA1-dependent Signal Transduction Pathway Is Essential for the Regulation of Cholesterol Homeostasis and Inflammatory Reactions.; Authors - Yan-Wei Hu
human	WB	J Lipid Res; VNN1 Promotes Atherosclerosis Progression in apoE Deficiency Mice Fed a High Fat High Cholesterol Diet.; Authors - Yan-Wei Hu
human	WB	PLoS One; Dihydrocapsaicin Attenuates Plaque Formation through a PPARγ/LXRα Pathway in apoE(-/-) Mice Fed a High-Fat/High-Cholesterol Diet.; Authors - Hu Yan-Wei YW
mouse	WB	J Ethnopharmacol; Effect of Si-Miao-Yong-An decoction on the differentiation of monocytes, macrophages, and regulatory T cells in ApoE-/- mice.; Authors - Xin-Nong Chen