Anticorps Polyclonal de lapin anti-c-SRC

c-SRC Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris, Hamster

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

65

N° de cat : 11097-1-AP

Synonymes

ASV, c SRC, p60 Src, pp60c src, Proto oncogene c Src, SRC, SRC1

---

Galerie de données de validation

• 

  WB analysis using 11097-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11097-1-AP (SRC antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11097-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11097-1-AP (SRC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 11097-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 11097-1-AP (SRC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of CHO using 11097-1-AP

  CHO cells were subjected to SDS PAGE followed by western blot with 11097-1-AP (SRC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 11097-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11097-1-AP (SRC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 11097-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 11097-1-AP (SRC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 11097-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 11097-1-AP (SRC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 11097-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 11097-1-AP (SRC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of SH-SY5Y using 11097-1-AP

  IP Result of anti-SRC (IP:11097-1-AP, 5ug; Detection:11097-1-AP 1:1000) with SH-SY5Y cells lysate 2900ug.

• 

  IHC staining of human breast cancer using 11097-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11097-1-AP (c-SRC Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11097-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11097-1-AP (c-SRC Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 11097-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11097-1-AP (c-SRC Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 11097-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11097-1-AP (c-SRC Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 11097-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 11097-1-AP (c-SRC Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 11097-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 11097-1-AP (c-SRC Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of A431 using 11097-1-AP

  Immunofluorescent analysis of A431 cells using 11097-1-AP (SRC antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of SH-SY5Y using 11097-1-AP

  1X10^6 SH-SY5Y cells were stained with 0.2ug c-SRC antibody (11097-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

---

Applications testées

• Résultats positifs en WB: cellules HUVEC, cellules A431, cellules CHO, cellules COLO 320, cellules Jurkat, cellules SH-SY5Y, tissu cérébral de souris
• Résultats positifs en IP: cellules SH-SY5Y
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu cérébral humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A431
• Résultats positifs en cytométrie: cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 55 publications below
• IHC: See 8 publications below
• IF: See 1 publications below
• IP: See 4 publications below

Informations sur le produit

11097-1-AP cible c-SRC dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris, Hamster

• Réactivité: Humain, rat, souris, Hamster
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: c-SRC Protéine recombinante Ag1575
• Nom complet: v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
• Masse moléculaire calculée: 60 kDa
• Poids moléculaire observé: 55 kDa, 60 kDa
• Numéro d’acquisition GenBank: BC011566
• Symbole du gène: SRC
• Identification du gène (NCBI): 6714
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SRC, also named as SRC1 and p60-Src, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, SRC plays an important role in osteoclastic bone resorption. It promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase. SRC can be detected as 60 kDa and 55 kDa (PMID: 15047935, 21461171).

Publications

Species	Application	Title
human	WB	Nat Genet; Pancreatic cancer risk variant in LINC00673 creates a miR-1231 binding site and interferes with PTPN11 degradation.; Authors - Jian Zheng
mouse	WB,IP	Cell Stem Cell; Amino acid catabolism regulates hematopoietic stem cell proteostasis via a GCN2-eIF2α axis.; Authors - Changzheng Li
human	WB	Sci Adv; Platelet P-selectin initiates cross-presentation and dendritic cell differentiation in blood monocytes.; Authors - Patrick Han
		J Cell Biol; Metalloproteinase MT1-MMP islets act as memory devices for podosome reemergence.; Authors - Karim El Azzouzi
human		Cell Death Differ; The E3 ubiquitin ligase TRIM7 suppressed hepatocellular carcinoma progression by directly targeting Src protein.; Authors - Lihui Zhu
mouse	WB	Haematologica; NLRP3 regulates platelet integrin αIIbβ3 outside-in signaling, hemostasis and arterial thrombosis.; Authors - Jianlin Qiao

Produits associés

c-Cbl Polyclonal antibody

25818-1-AP

CRK Polyclonal antibody

16685-1-AP

EGFR (N-terminal) Polyclonal antibody

22542-1-AP

STAT3 Polyclonal antibody

51076-2-AP

CRK Polyclonal antibody

16685-1-AP