Anticorps Polyclonal de lapin anti-SRF

• Phare
• Validé par KD/KO

SRF Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications(28)

Avis (1)

N° de cat : 16821-1-AP

Synonymes

Serum response factor, MCM1

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Galerie de données de validation

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  WB analysis of MCF-7 using 16821-1-AP

  WB result of SRF antibody (16821-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SRF transfected MCF-7 cells.

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  WB analysis using 16821-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16821-1-AP (SRF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16821-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16821-1-AP (SRF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 16821-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16821-1-AP (SRF antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 16821-1-AP

  IP result of anti-SRF (IP:16821-1-AP, 3ug; Detection:16821-1-AP 1:1000) with HeLa cells lysate 3000ug.

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  IHC staining of mouse heart using 16821-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16821-1-AP (SRF antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 16821-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16821-1-AP (SRF antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 16821-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using SRF antibody (16821-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HEK-293, cellules HeLa, cellules K-562, cellules MCF-7, cellules NIH/3T3
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu cardiaque de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 20 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• IP: See 3 publications below
• CoIP: See 3 publications below
• ChIP: See 2 publications below

Informations sur le produit

16821-1-AP cible SRF dans les applications de WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SRF Protéine recombinante Ag10386
• Nom complet: serum response factor (c-fos serum response element-binding transcription factor)
• Masse moléculaire calculée: 508 aa, 52 kDa
• Poids moléculaire observé: 65-70 kDa, 40 kDa
• Numéro d’acquisition GenBank: BC048211
• Symbole du gène: SRF
• Identification du gène (NCBI): 6722
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Serum response factor (SRF) is a transcription factor that binds the serum response element (SRE), a sequence that mediates the transient response of many cellular genes to growth stimulation. SRF is required for cardiac differentiation and maturation due to the role in cardiac cell growth and muscle gene regulation. The full-length SRF protein is 67 kDa and molecular weight of cleaved fragment is 50 kDa and 20 kDa (PMID: 21769134).

Publications

Species	Application	Title
human	WB	Exp Mol Med; Helicobacter pylori CagA-mediated ether lipid biosynthesis promotes ferroptosis susceptibility in gastric cancer; Authors - Yanmei Peng ; KD Validated
human	WB	Cell Mol Life Sci; Crip2 affects vascular development by fine-tuning endothelial cell aggregation and proliferation; Authors - Shuaiqi Yang
human	IP	Am J Respir Cell Mol Biol; SRF Expression in Excess Permits a Dual Contractile-Proliferative Phenotype of Airway Smooth Muscle; Authors - Rui Sun
	WB	Cell Physiol Biochem; Galectin-3- Mediated Transdifferentiation of Pulmonary Artery Endothelial Cells Contributes to Hypoxic Pulmonary Vascular Remodeling.; Authors - Li Zhang
mouse	WB	Stem Cell Res Ther; Activin B-activated Cdc42 signaling plays a key role in regulating adipose-derived mesenchymal stem cells-mediated skin wound healing.; Authors - Simin Huang
human	WB	Am J Physiol Lung Cell Mol Physiol; Myocardin regulates fibronectin expression and secretion from human pleural mesothelial cells; Authors - Tsuyoshi Sakai

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Carmen (Verified Customer) (11-29-2024)

Works well IHC set up on the Leica Bond

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Murine pancreatic tumour

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