Anticorps Polyclonal de lapin anti-SRGAP1

SRGAP1 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 13252-1-AP

Synonymes

ARHGAP13, KIAA1304, SRGAP1

---

Galerie de données de validation

• 

  WB analysis using 13252-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13252-1-AP (SRGAP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Apoptosised HeLa using 13252-1-AP

  Apoptosised HeLa cells were subjected to SDS PAGE followed by western blot with 13252-1-AP (SRGAP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 13252-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13252-1-AP (SRGAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 13252-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13252-1-AP (SRGAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 13252-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 13252-1-AP (SRGAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 13252-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13252-1-AP (SRGAP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 13252-1-AP

  IP Result of anti-SRGAP1 (IP:13252-1-AP, 4ug; Detection:13252-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

• 

  IHC staining of human liver cancer using 13252-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13252-1-AP (SRGAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 13252-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13252-1-AP (SRGAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 13252-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13252-1-AP (SRGAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 13252-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13252-1-AP (SRGAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 13252-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13252-1-AP (SRGAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 13252-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13252-1-AP (SRGAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HeLa apoptosées, cellules SH-SY5Y, tissu cérébral de souris, tissu rénal de souris
• Résultats positifs en IP: tissu rénal de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu rénal humain, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

13252-1-AP cible SRGAP1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SRGAP1 Protéine recombinante Ag4104
• Nom complet: SLIT-ROBO Rho GTPase activating protein 1
• Masse moléculaire calculée: 1085 aa, 124 kDa
• Poids moléculaire observé: 124 kDa
• Numéro d’acquisition GenBank: BC029919
• Symbole du gène: SRGAP1
• Identification du gène (NCBI): 57522
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB,IF,IP	J Am Soc Nephrol; SRGAP1 Controls Small Rho GTPases To Regulate Podocyte Foot Process Maintenance.; Authors - Manuel Rogg
human	WB	J Exp Clin Cancer Res; srGAP1 mediates the migration inhibition effect of Slit2-Robo1 in colorectal cancer.; Authors - Yuyang Feng
human	WB,IF	Cell Rep; A proliferative to invasive switch is mediated by srGAP1 downregulation through the activation of TGF-β2 signaling; Authors - Chandrani Mondal