Anticorps Polyclonal de lapin anti-Spermidine synthase

Spermidine synthase Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

12

N° de cat : 19858-1-AP

Synonymes

PAPT, SPDSY, Spermidine synthase, SPS1, SRM, SRML1

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Galerie de données de validation

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  WB analysis using 19858-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19858-1-AP (Spermidine synthase antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 19858-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19858-1-AP (Spermidine synthase antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of LNCaP using 19858-1-AP

  LNCaP cells were subjected to SDS PAGE followed by western blot with 19858-1-AP (Spermidine synthase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 19858-1-AP

  IP Result of anti-Spermidine synthase (IP:19858-1-AP, 4ug; Detection:19858-1-AP 1:500) with mouse liver tissue lysate 4000ug.

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  IHC staining of human kidney using 19858-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 19858-1-AP (Spermidine synthase antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 19858-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19858-1-AP (Spermidine synthase antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver cancer using 19858-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19858-1-AP (Spermidine synthase antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 19858-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Spermidine synthase antibody (19858-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules LNCaP, cellules PC-3, cellules RAW 264.7, tissu cérébral de souris, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IP: tissu hépatique de souris
• Résultats positifs en IHC: tissu rénal humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 11 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

19858-1-AP cible Spermidine synthase dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Spermidine synthase Protéine recombinante Ag13936
• Nom complet: spermidine synthase
• Masse moléculaire calculée: 302 aa, 34 kDa
• Poids moléculaire observé: 34 kDa
• Numéro d’acquisition GenBank: BC000309
• Symbole du gène: SRM
• Identification du gène (NCBI): 6723
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Spermidine synthase is an enzyme that catalyzes the transfer of the propylamine group from S-adenosylmethioninamine to putrescine in the biosynthesis of spermidine. The polyamines putrescine, spermine, and spermidine are ubiquitous polycationic mediators of cell growth and differentiation. Spermidine synthases catalyze the production of the linear triamine, spermidine, from putrescine. They utilize decarboxylated S-adenosylmethionine (dc-SAM), a universal cofactor of aminopropyltransferases, as a donor of the aminopropyl moiety (PMID:31134111).

Publications

Species	Application	Title
mouse	WB	J Clin Invest; Aberrant AZIN2 and polyamine metabolism precipitates tau neuropathology.; Authors - Leslie A Sandusky-Beltran
human	WB,IF	Nat Commun; Macrophage ABHD5 promotes colorectal cancer growth by suppressing spermidine production by SRM.; Authors - Hongming Miao
human	WB	Cancer Sci; The miR-1908/SRM regulatory axis contributes to extracellular vesicle secretion in prostate cancer.; Authors - Fumihiko Urabe
mouse	WB	J Proteomics; Proteomic analysis revealed different responses to hypergravity of soleus and extensor digitorum longus muscles in mice.; Authors - Takashi Ohira
mouse	WB	Am J Physiol Cell Physiol; The Regulation of Polyamine Pathway Proteins in Models of Skeletal Muscle Hypertrophy and Atrophy - a potential role for mTORC1.; Authors - Michael Tabbaa
	IHC	Biol Reprod; Progesterone and interferon tau regulate expression of polyamine enzymes during the ovine peri-implantation period.; Authors - Katherine M Halloran