Anticorps Polyclonal de lapin anti-SRP9

• Phare
• Validé par KD/KO

SRP9 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 11195-1-AP

Synonymes

ALURBP, SRP9

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Galerie de données de validation

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  WB analysis of HepG2 using 11195-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11195-1-AP (SRP9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11195-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11195-1-AP (SRP9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11195-1-AP

  WB result of SRP9 antibody (11195-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SRP9 transfected HeLa cells.

• 

  WB analysis of A2780 using 11195-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 11195-1-AP (SRP9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SKOV-3 using 11195-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 11195-1-AP (SRP9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 11195-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 11195-1-AP (SRP9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11195-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11195-1-AP (SRP9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11195-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11195-1-AP (SRP9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11195-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11195-1-AP (SRP9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 11195-1-AP

  IP Result of anti-SRP9 (IP:11195-1-AP, 3ug; Detection:11195-1-AP 1:500) with HepG2 cells lysate 3600ug.

• 

  IHC staining of human prostate cancer using 11195-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11195-1-AP (SRP9 antibody) at dilution of 1:100 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules A2780, cellules HeLa, cellules HuH-7, cellules MCF-7, cellules SKOV-3, tissu de côlon de souris
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu de cancer de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 1 publications below
• IP: See 2 publications below

Informations sur le produit

11195-1-AP cible SRP9 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SRP9 Protéine recombinante Ag1683
• Nom complet: signal recognition particle 9kDa
• Masse moléculaire calculée: 10 kDa
• Poids moléculaire observé: 10 kDa
• Numéro d’acquisition GenBank: BC015094
• Symbole du gène: SRP9
• Identification du gène (NCBI): 6726
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Signal recognition particle 9(SRP9) is component of the signal recognition particle (SRP), which is a ribonucleoprotein complex that mediates the targeting of proteins to the rough endoplasmic reticulum (ER). SRP9 form a heterodimer with SRP14, which involves in arrest of the elongation of the nescent chains during targeting to ensure efficient translocation of the preprotein.

Publications

Species	Application	Title
human	WB	Cell; Exosome RNA Unshielding Couples Stromal Activation to Pattern Recognition Receptor Signaling in Cancer.; Authors - Barzin Y Nabet ; KD Validated
human	WB,IP	Nucleic Acids Res; Comprehensive analysis of the BC200 ribonucleoprotein reveals a reciprocal regulatory function with CSDE1/UNR.; Authors - Evan P Booy
	WB	Nucleic Acids Res; The pioneer round of translation ensures proper targeting of ER and mitochondrial proteins.; Authors - Joori Park
human	WB,IP	Nucleic Acids Res; Implication of the SMN complex in the biogenesis and steady state level of the signal recognition particle.; Authors - Piazzon Nathalie N
human	WB	J Proteome Res; Proteomic expression analysis of surgical human colorectal cancer tissues: up-regulation of PSB7, PRDX1, and SRP9 and hypoxic adaptation in cancer.; Authors - Rho Jung-hyun JH
human	WB	J Biol Chem; The noncoding RNA BC200 associates with polysomes to positively regulate mRNA translation in tumor cells.; Authors - Evan P Booy