Anticorps Polyclonal de lapin anti-SRPK1

SRPK1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 14073-1-AP

Synonymes

SFRS protein kinase 1, SFRSK1, SR protein specific kinase 1, SRPK1

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Galerie de données de validation

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  WB analysis using 14073-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14073-1-AP (SRPK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14073-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14073-1-AP (SRPK1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 14073-1-AP

  IP result of anti-SRPK1(IP:14073-1-AP, 4ug; Detection:14073-1-AP 1:500) with mouse testis tissue lysate 2040 ug.

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  IHC staining of human breast cancer using 14073-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14073-1-AP (SRPK1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 14073-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14073-1-AP (SRPK1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human testis using 14073-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14073-1-AP (SRPK1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 14073-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14073-1-AP (SRPK1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 14073-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14073-1-AP (SRPK1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 14073-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14073-1-AP (SRPK1 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of SH-SY5Y using 14073-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 14073-1-AP (SRPK1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of MCF-7 using 14073-1-AP

  1X10^6 MCF-7 cells were stained with 0.2ug SRPK1 antibody (14073-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat, cellules L02, tissu testiculaire de souris
• Résultats positifs en IP: tissu testiculaire de souris,
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules SH-SY5Y
• Résultats positifs en cytométrie: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

14073-1-AP cible SRPK1 dans les applications de WB, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SRPK1 Protéine recombinante Ag5149
• Nom complet: SFRS protein kinase 1
• Masse moléculaire calculée: 92.4 kDa
• Poids moléculaire observé: 72-95 kDa
• Numéro d’acquisition GenBank: BC038292
• Symbole du gène: SRPK1
• Identification du gène (NCBI): 6732
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SRPK1 (serine-arginine protein kinase 1) is a protein kinase that specifically phosphorylates proteins containing serine-arginine-rich domains. Its substrates include a family of SR proteins that are key regulators of mRNA AS (alternative splicing). SRPK1 may be localized both in the cytoplasm and nucleus and translocates between these compartments under various conditions (e.g. cell cycle and stress). It is able to phosphorylate SR proteins both in cytoplasm and nucleus (the significance of which remains incompletely understood) and thus regulate the activity of these proteins by affecting their ability to bind other proteins and/or RNA as well as their intracellular localization.(PMID:22817743). This protein has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
	WB,CoIP	Cell Metab; DRAK2 aggravates nonalcoholic fatty liver disease progression through SRSF6-associated RNA alternative splicing.; Authors - Yufeng Li
human	WB	Cell Chem Biol; Novel RNA-Affinity Proteogenomics Dissects Tumor Heterogeneity for Revealing Personalized Markers in Precision Prognosis of Cancer.; Authors - Li Wang
human	WB	J Cancer; Clinical and functional significance of CHK1-S, an alternatively spliced isoform of the CHK1 gene, in hepatocellular carcinoma.; Authors - Guanghui Hu
mouse	IF	BMC Cancer; MiR-659-3p inhibits osteosarcoma progression and metastasis by inhibiting cell proliferation and invasion via targeting SRPK1; Authors - Yubao Gong