Anticorps Polyclonal de lapin anti-SRPK2

• Phare
• Validé par KD/KO

SRPK2 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 25417-1-AP

Synonymes

SFRS protein kinase 2, SFRSK2, SR protein specific kinase 2, SRPK2

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Galerie de données de validation

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  WB analysis of HeLa using 25417-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25417-1-AP (SRPK2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 25417-1-AP

  WB result of SRPK2 antibody (25417-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-SRPK2 transfected HeLa cells.

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  WB analysis of HeLa using 25417-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25417-1-AP (SRPK2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 25417-1-AP

  IP result of anti-SRPK2 (IP:25417-1-AP, 4ug; Detection:25417-1-AP 1:300) with HeLa cells lysate 3200ug.

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  IP experiment of HeLa using 25417-1-AP

  IP result of anti-SRPK2 (IP:25417-1-AP, 4ug; Detection:25417-1-AP 1:300) with HeLa cells lysate 3200ug.

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  IHC staining of mouse testis using 25417-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25417-1-AP (SRPK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 25417-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25417-1-AP (SRPK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 25417-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25417-1-AP (SRPK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 25417-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25417-1-AP (SRPK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 25417-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25417-1-AP (SRPK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa,
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu testiculaire de souris, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

25417-1-AP cible SRPK2 dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SRPK2 Protéine recombinante Ag17885
• Nom complet: SFRS protein kinase 2
• Masse moléculaire calculée: 688 aa, 78 kDa
• Poids moléculaire observé: 105-110 kDa
• Numéro d’acquisition GenBank: BC035214
• Symbole du gène: SRPK2
• Identification du gène (NCBI): 6733
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SRPK2, also named as serine/arginine protein kinase 2 and SFRSK2, belongs to SR protein-specific kinase family which phosphorylate the serine/arginine domain containing proteins in the nuclear speckles to mediate pre-mRNA splicing (PMID: 22854045). In addition to its major role as kinase for the phosphorylation of SR proteins, SRPK2 is involved in cell cycle regulation and cell apoptosis (PMID: 19592491). SRPK2 promotes the proliferation and migration of the colon cancer cells. It has been reported to be regulated in several cancer types (PMID: 27041240). It has 2 isoforms with the calculated molecular mass of 77-79 kDa, and the apparent molecular mass of 105-115 kDa due to posttranslational modification (PMID: 12134018, 16122776).

Publications

Species	Application	Title
human	WB,IF	Cancer Discov; Stress Granules determine the Development of Obesity-associated Pancreatic Cancer.; Authors - Guillaume Fonteneau

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