Anticorps Polyclonal de lapin anti-SRPX2

• Phare
• Validé par KD/KO

SRPX2 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF

Conjugaison

Non conjugué

Publications

6

N° de cat : 11845-1-AP

Synonymes

BPP, CBPS, PMGX, RESDX, SRPUL, SRPX2

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Galerie de données de validation

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  WB analysis of human placenta using 11845-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 11845-1-AP (SRPX2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11845-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11845-1-AP (SRPX2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 11845-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 11845-1-AP (SRPX2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11845-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11845-1-AP (SRPX2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 11845-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 11845-1-AP (SRPX2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 11845-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 11845-1-AP (SRPX2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 11845-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 11845-1-AP (SRPX2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 11845-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 11845-1-AP (SRPX2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 11845-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 11845-1-AP (SRPX2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 11845-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 11845-1-AP (SRPX2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 11845-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 11845-1-AP (SRPX2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 11845-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 11845-1-AP (SRPX2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 11845-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 11845-1-AP (SRPX2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 11845-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 11845-1-AP (SRPX2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human heart using 11845-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 11845-1-AP (SRPX2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 11845-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 11845-1-AP (SRPX2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 11845-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11845-1-AP (SRPX2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 11845-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11845-1-AP (SRPX2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of rat brain using 11845-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using SRPX2 antibody (11845-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of rat brain using 11845-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using SRPX2 antibody (11845-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu placentaire humain, cellules HepG2, tissu cérébral humain, tissu rénal humain
• Résultats positifs en IHC: tissu placentaire humain, tissu cardiaque humain, tissu cérébral humain, tissu cutané humain, tissu pulmonaire humain, tissu rénal humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu cérébral de rat,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 2 publications below

Informations sur le produit

11845-1-AP cible SRPX2 dans les applications de WB, IHC, IF et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SRPX2 Protéine recombinante Ag2516
• Nom complet: sushi-repeat-containing protein, X-linked 2
• Masse moléculaire calculée: 465 aa, 53 kDa
• Poids moléculaire observé: 53 kDa
• Numéro d’acquisition GenBank: BC020733
• Symbole du gène: SRPX2
• Identification du gène (NCBI): 27286
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SRPX2 (sushi-repeat-containing protein, X-linked 2) is a secreted protein expressed in neurons of the human adult brain, including the rolandic area (PMID: 20874700). Firstly described as sushi-repeat protein up-regulated in leukemia (SPRUL) in leukemia cells with dysregulated expression at the transcriptional level, SRPX2 has been recently shown as a multifunctional protein. SRPX2 is a ligand for uPAR, the urokinase-type plasminogen activator (uPA) receptor (PMID: 18718938). It is involved in seizure disorders, angiogenesis and cellular adhesion (PMID: 22242148; 19667118; 16497722). The involvement of SRPX2 in disorders of language cortex and cognition suggests it has an important role in the perisylvian region critical for language development (PMID: 16497722).

Publications

Species	Application	Title
human	WB	Theranostics; Local administration of liposomal-based Srpx2 gene therapy reverses pulmonary fibrosis by blockading fibroblast-to-myofibroblast transition.; Authors - Qi Wang ; KD Validated
human	WB	Hum Mol Genet; Epileptic and developmental disorders of the speech cortex: ligand/receptor interaction of wild-type and mutant SRPX2 with the plasminogen activator receptor uPAR.; Authors - Royer-Zemmour Barbara B
mouse	WB	FASEB J; Sushi repeat protein X-linked 2, a novel mediator of angiogenesis.; Authors - Miljkovic-Licina Marijana M
human	IHC	Front Cell Dev Biol; The Landscape of the Tumor Microenvironment in Skin Cutaneous Melanoma Reveals a Prognostic and Immunotherapeutically Relevant Gene Signature.; Authors - Sitong Zhou
mouse	WB	Int J Mol Sci; The Role of Lysosomes in a Broad Disease-Modifying Approach Evaluated across Transgenic Mouse Models of Alzheimer's Disease and Parkinson's Disease and Models of Mild Cognitive Impairment.; Authors - Jeannie Hwang
human	WB, IHC	Int J Clin Exp Pathol; SRPX2 promotes cell migration and invasion via FAK dependent pathway in pancreatic cancer.; Authors - Zhenyuan Gao