Anticorps Polyclonal de lapin anti-SRRM1

• Phare
• Validé par KD/KO

SRRM1 Polyclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 12822-1-AP

Synonymes

160 KD, POP101, SRM160, SRRM1

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Galerie de données de validation

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  WB analysis of mouse liver using 12822-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12822-1-AP (SRRM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 12822-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12822-1-AP (SRRM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 12822-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12822-1-AP (SRRM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12822-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12822-1-AP (SRRM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of UV treated HEK-293 using 12822-1-AP

  UV treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 12822-1-AP (SRRM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 12822-1-AP

  IP Result of anti-SRRM1 (IP:12822-1-AP, 4ug; Detection:12822-1-AP 1:300) with HeLa cells lysate 3200ug.

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules HeLa, cellules Jurkat, HEK-293 traitées aux UV
• Résultats positifs en IP: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

12822-1-AP cible SRRM1 dans les applications de WB, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SRRM1 Protéine recombinante Ag3513
• Nom complet: serine/arginine repetitive matrix 1
• Masse moléculaire calculée: 904 aa, 102 kDa
• Poids moléculaire observé: 120-160 kDa
• Numéro d’acquisition GenBank: BC036187
• Symbole du gène: SRRM1
• Identification du gène (NCBI): 10250
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SRRM1, also named as SRM160 and POP101, contains one PWI domain and belongs to the splicing factor SR family. As part of pre- and post-splicing multiprotein mRNP complexes, SRRM1 is involved in numerous pre-mRNA processing events. It promotes constitutive and exonic splicing enhancer (ESE)-dependent splicing activation by bridging together sequence-specific (SR family proteins, SFRS4, SFRS5 and TRA2B/SFRS10) and basal snRNP (SNRP70 and SNRPA1) factors of the spliceosome. SRRM1 stimulates mRNA 3'-end cleavage independently of the formation of an exon junction complex. It binds both pre-mRNA and spliced mRNA 20-25 nt upstream of exon-exon junctions. SRRM1 binds RNA and DNA with low sequence specificity and has similar preference for either double- or single-stranded nucleic acid substrates. SRRM1, a protein of high molecular weight(120-160kd), has a heavy glycosylation level and is normally highly phosphorylated. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human SRRM1.

Publications

Species	Application	Title
human	WB	Cell Host Microbe; Quantitative phosphoproteomics reveals extensive cellular reprogramming during HIV-1 entry.; Authors - Wojcechowskyj Jason A JA ; KD Validated
human	WB	Biochim Biophys Acta Mol Cell Res; Cleaved PGAM5 dephosphorylates nuclear serine/arginine-rich proteins during mitophagy.; Authors - Taiki Baba
human	CoIP	Tissue Cell; SRRM1 promotes the proliferation, migration, and invasion of hepatocellular carcinoma cells by regulating the JAK/STAT signaling pathway; Authors - Xiaoyan Song