Anticorps Monoclonal anti-SRY

SRY Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

1

CloneNo.

2E9A4

N° de cat : 67349-1-Ig

Synonymes

sex determining region Y, SRY, TDF, TDY, Testis determining factor

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Galerie de données de validation

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  WB analysis using 67349-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67349-1-Ig (SRY antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. SRY negative cell line HeLa cell line was served as negative control.

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  WB analysis of LNCaP using 67349-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67349-1-Ig (SRY antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of DU 145 using 67349-1-Ig

  DU 145 cells were subjected to SDS PAGE followed by western blot with 67349-1-Ig (SRY antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules DU 145, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

67349-1-Ig cible SRY dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SRY Protéine recombinante Ag12610
• Nom complet: sex determining region Y
• Masse moléculaire calculée: 204 aa, 24 kDa
• Poids moléculaire observé: 25-30 kDa
• Numéro d’acquisition GenBank: BC074924
• Symbole du gène: SRY
• Identification du gène (NCBI): 6736
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SRY (sex-determining region Y protein) is a tran-scriptional activator required for male sex determination in mammals. This protein, also referred to as testis-determining factor (TDF), is an HMG box protein that initiates the formation of testis from undifferentiated gonad. The DNA-binding activity of SRY is required for normal testis formation. This DNA-binding activity is thought to be regulated by PKA, which phosphorylates SRY in vivo. Mutations in SRY have been associated with 46,XY gonadal dysgenesis, in which the gonads fail to develop in XY phenotypic females.

Publications

Species	Application	Title
human	WB	Cell Rep; Recruitment of DNA to tumor-derived microvesicles.; Authors - James W Clancy