Anticorps Polyclonal de lapin anti-SSB

SSB Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 11720-1-AP

Synonymes

La, La autoantigen, La ribonucleoprotein, LARP3, Lupus La protein

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Galerie de données de validation

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  WB analysis of Jurkat using 11720-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11720-1-AP (SSB Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11720-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11720-1-AP (SSB Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11720-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11720-1-AP (SSB Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 11720-1-AP

  IP result of anti-SSB (IP:11720-1-AP, 4ug; Detection:11720-1-AP 1:300) with Jurkat cells lysate 3600ug.

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  IF Staining of HeLa using 11720-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SSB antibody (11720-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HEK-293, cellules HeLa
• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below

Informations sur le produit

11720-1-AP cible SSB dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SSB Protéine recombinante Ag2294
• Nom complet: Sjogren syndrome antigen B (autoantigen La)
• Masse moléculaire calculée: 408 aa, 47 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: BC001289
• Symbole du gène: SSB
• Identification du gène (NCBI): 6741
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Cell Chem Biol; Novel RNA-Affinity Proteogenomics Dissects Tumor Heterogeneity for Revealing Personalized Markers in Precision Prognosis of Cancer.; Authors - Li Wang
human	WB	EMBO Rep; Human METTL16 is a N6-methyladenosine (m6A) methyltransferase that targets pre-mRNAs and various non-coding RNAs.; Authors - Ahmed S Warda
human	WB	Oxid Med Cell Longev; The Formation of Melanocyte Apoptotic Bodies in Vitiligo and the Relocation of Vitiligo Autoantigens under Oxidative Stress.; Authors - Jun Tian
human	WB	J Virol; Inhibition of Hepatitis B Virus Gene Expression and Replication by Hepatocyte Nuclear Factor 6.; Authors - Ruidong Hao
human	WB	Cell Mol Biol Lett; Salivary gland protective and antiinflammatory effects of genistein in Sjögren's syndrome by inhibiting Xist/ACSL4-mediated ferroptosis following binding to estrogen receptor-alpha; Authors - Tianjiao Mao

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