Anticorps Polyclonal de lapin anti-SSBP1

• Phare
• Validé par KD/KO

SSBP1 Polyclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ChIP, ELISA

Conjugaison

Non conjugué

Publications

23

N° de cat : 12212-1-AP

Synonymes

Mt SSB, MtSSB, PWP1 interacting protein 17, SSBP, SSBP1

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Galerie de données de validation

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  WB analysis of A549 using 12212-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12212-1-AP (SSBP1 antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

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  WB analysis using 12212-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12212-1-AP (SSBP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 12212-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 12212-1-AP (SSBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 12212-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12212-1-AP (SSBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat pancreas using 12212-1-AP

  rat pancreas tissue were subjected to SDS PAGE followed by western blot with 12212-1-AP (SSBP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of A375 using 12212-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A375 cells using 12212-1-AP (SSBP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules A375, cellules Caco-2, tissu pancréatique de rat, tissu pancréatique de souris
• Résultats positifs en IF: cellules A375

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 20 publications below
• IHC: See 3 publications below
• IF: See 2 publications below
• ChIP: See 1 publications below

Informations sur le produit

12212-1-AP cible SSBP1 dans les applications de WB, IHC, IF, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SSBP1 Protéine recombinante Ag2860
• Nom complet: single-stranded DNA binding protein 1
• Masse moléculaire calculée: 148 aa, 17 kDa
• Poids moléculaire observé: 17 kDa
• Numéro d’acquisition GenBank: BC000895
• Symbole du gène: SSBP1
• Identification du gène (NCBI): 6742
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SSBP1 is a housekeeping gene involved in mitochondrial biogenesis. it binds preferentially and cooperatively to ss-DNA in the maintainance of genome stability. SSBP1 acted as a homotetramer to stabilize the displaced single strand of the normal and expanded displacement loop (D loop) during mtDNA replication, thus preventing formation of secondary single-stranded DNA structures, which could stop the gamma-DNA polymerase

Publications

Species	Application	Title
human	WB	Cell; MOF Acetyl Transferase Regulates Transcription and Respiration in Mitochondria.; Authors - Aindrila Chatterjee
human	WB	Nat Cell Biol; Mitochondrial RNA granules are fluid condensates positioned by membrane dynamics.; Authors - Timo Rey
human	WB	Mol Cell; Single-Molecule Analysis of mtDNA Replication Uncovers the Basis of the Common Deletion.; Authors - Aaron F Phillips
		Sci Adv; The mitochondrial single-stranded DNA binding protein is essential for initiation of mtDNA replication.; Authors - Min Jiang ; KO Validated
human	IHC	Nucleic Acids Res; Top3α is the replicative topoisomerase in mitochondrial DNA replication.; Authors - Anu Hangas
human	WB	Brain; Heterozygous SSBP1 start loss mutation co-segregates with hearing loss and the m.1555A>G mtDNA variant in a large multigenerational family.; Authors - Peter J Kullar