Anticorps Polyclonal de lapin anti-KRAP/SSFA2

• Phare
• Validé par KD/KO

KRAP/SSFA2 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF-P, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(8)

N° de cat : 14157-1-AP

Synonymes

SSFA2, ITPRID2, CS-1, CS1, CS 1

---

Galerie de données de validation

• 

  WB analysis using 14157-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14157-1-AP (KRAP/SSFA2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14157-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14157-1-AP (KRAP/SSFA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 14157-1-AP

  IP result of anti-KRAP/SSFA2 (IP:14157-1-AP, 4ug; Detection:14157-1-AP 1:500) with mouse testis tissue lysate 4000ug.

• 

  IHC staining of human stomach using 14157-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 14157-1-AP (KRAP/SSFA2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse pancreas using 14157-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using KRAP/SSFA2 antibody (14157-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse pancreas using 14157-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using KRAP/SSFA2 antibody (14157-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: tissu pulmonaire de souris, tissu pulmonaire de rat, tissu testiculaire de souris
• Résultats positifs en IP: tissu testiculaire de souris
• Résultats positifs en IHC: tissu d'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu pancréatique de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Informations sur le produit

14157-1-AP cible KRAP/SSFA2 dans les applications de WB, IHC, IF-P, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: canin, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KRAP/SSFA2 Protéine recombinante Ag5339
• Nom complet: sperm specific antigen 2
• Masse moléculaire calculée: 138 kDa
• Poids moléculaire observé: 180 kDa
• Numéro d’acquisition GenBank: BC028706
• Symbole du gène: SSFA2
• Identification du gène (NCBI): 6744
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KRAP (Ki-ras-induced actin-interacting protein), also known as SSFA2, CS1 or SPAG13, is a 1,259 amino acid cytoplasmic protein associated with filamentous-actin (F-actin). KRAP is strongly expressed in pancreas and testis. It is also present in colon cancer cell lines, and the expression is up-regulated by activated K-Ras. KRAP-deficient (KO) mice display decreased adiposity and improved glucose metabolism, implicating KRAP as a potential target for the prevention or treatment of obesity and related diseases. KRAP has also been suggested to be a potential target for cancer. This antibody recognizes endogenous KRAP, which migrates with an apparent molecular mass of 180 kDa in SDS-PAGE. (PMID: 14673706; 21501587; 21873152)

Publications

Species	Application	Title
human	WB,IHC	Nat Commun; KRAP tethers IP3 receptors to actin and licenses them to evoke cytosolic Ca2+ signals.; Authors - Nagendra Babu Thillaiappan ; KD Validated
canine	WB, IF	J Cell Sci; Vimentin and the K-Ras-induced actin-binding protein control inositol-(1,4,5)-trisphosphate receptor redistribution during MDCK cell differentiation.; Authors - Dingli Florent F ; KD Validated
human	WB,IHC	J Proteomics; Relationship between phosphorylation of sperm-specific antigen and prognosis of lung adenocarcinoma.; Authors - Akiko Okayama
human	WB,IF,CoIP	Reprod Biol Endocrinol; A loss-of-function variant in SSFA2 causes male infertility with globozoospermia and failed oocyte activation.; Authors - Gelin Huang
human	WB	Front Immunol; Identification of a novel cuproptosis-related gene signature and integrative analyses in patients with lower-grade gliomas; Authors - Jia-hao Bao
human	WB	J Cell Sci; CREB regulates the expression of type 1 inositol 1,4,5-trisphosphate receptors; Authors - Vikas Arige

{{ptg:RelatedPrimaryAntibodies}}