Anticorps Polyclonal de lapin anti-TRAPB/SSR2

• Phare
• Validé par KD/KO

TRAPB/SSR2 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 10278-1-AP

Synonymes

HSD25, SSR beta, SSR2, TLAP, TRAP BETA, TRAPB, TRAPB/SSR2

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Galerie de données de validation

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  WB analysis using 10278-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10278-1-AP (TRAPB/SSR2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 10278-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10278-1-AP (TRAPB/SSR2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 10278-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10278-1-AP (TRAPB/SSR2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas using 10278-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 10278-1-AP (TRAPB/SSR2 antibody at dilution of 1:50.

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  IHC staining of human pancreas using 10278-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 10278-1-AP (TRAPB/SSR2 antibody at dilution of 1:50.

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  IF Staining of HepG2 using 10278-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TRAPB/SSR2 antibody (10278-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of MCF-7 using 10278-1-AP

  1X10^6 MCF-7 cells were stained with 0.2ug TRAPB/SSR2 antibody (10278-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules L02, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,
• Résultats positifs en cytométrie: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

10278-1-AP cible TRAPB/SSR2 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRAPB/SSR2 Protéine recombinante Ag0304
• Nom complet: signal sequence receptor, beta (translocon-associated protein beta)
• Masse moléculaire calculée: 20 kDa
• Poids moléculaire observé: 22-25 kDa
• Numéro d’acquisition GenBank: BC000341
• Symbole du gène: SSR2
• Identification du gène (NCBI): 6746
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TRAPB (translocon-associated protein subunit beta), also known as SSR2 (signal sequence receptor subunit beta), is a 22-kDa single-spanning membrane glycoprotein of the endoplasmic reticulum (ER) (PMID: 7789174). It is a part of the tetrameric TRAP complex (TRAP-alpha, TRAP-beta, TRAP-delta and TRAP-gamma) which functions in regulating the retention of ER resident proteins (PMID: 7916687). The TRAP complex is also involved in endoplasmic reticulum-associated degradation (ERAD) (PMID: 17380188).

Publications

Species	Application	Title
human	WB	Mol Cell; RNF126-Mediated Reubiquitination Is Required for Proteasomal Degradation of p97-Extracted Membrane Proteins.; Authors - Xianyan Hu
human	WB	Sci Adv; The translocon-associated protein (TRAP) complex regulates quality control of N-linked glycosylation during ER stress.; Authors - Chatchai Phoomak
human	WB	Diabetes; The Role of TRAPγ/SSR3 in Preproinsulin Translocation into the Endoplasmic Reticulum.; Authors - Xiaoxi Xu
rat	WB, IP	FASEB J; Deficient endoplasmic reticulum translocon-associated protein complex limits the biosynthesis of proinsulin and insulin.; Authors - Yumeng Huang ; KD Validated
human	WB,IHC	J Cell Mol Med; Overexpression of SSR2 promotes proliferation of liver cancer cells and predicts prognosis of patients with hepatocellular carcinoma.; Authors - Fengsui Chen ; KD Validated
human	IHC	J Immunol Res; Endoplasmic Reticulum Stress-Related Signature for Predicting Prognosis and Immune Features in Hepatocellular Carcinoma; Authors - Genhao Zhang