Anticorps Polyclonal de lapin anti-SSSCA1

• Phare
• Validé par KD/KO

SSSCA1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 12273-1-AP

Synonymes

Autoantigen p27, p27, SSSCA1, ZNRD2

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Galerie de données de validation

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  WB analysis of HeLa using 12273-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12273-1-AP (SSSCA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 12273-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12273-1-AP (SSSCA1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 12273-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12273-1-AP (SSSCA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12273-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12273-1-AP (SSSCA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 12273-1-AP

  IP Result of anti-SSSCA1 (IP:12273-1-AP, 4ug; Detection:12273-1-AP 1:1000) with HeLa cells lysate 2000ug.

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  IHC staining of mouse testis using 12273-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12273-1-AP (SSSCA1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 12273-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12273-1-AP (SSSCA1 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 12273-1-AP

  Immunofluorescent analysis of Hela cells, using SSSCA1 antibody 12273-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of HeLa using 12273-1-AP

  1X10^6 HeLa cells were stained with 0.2 ug SSSCA1 antibody (12273-1-AP) and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1500 (red), or stained with 0.2 ug isotype control and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1500 (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules NIH/3T3
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu testiculaire de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below

Informations sur le produit

12273-1-AP cible SSSCA1 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SSSCA1 Protéine recombinante Ag2916
• Nom complet: Sjogren syndrome/scleroderma autoantigen 1
• Masse moléculaire calculée: 199 aa, 21 kDa
• Poids moléculaire observé: 27 kDa
• Numéro d’acquisition GenBank: BC014791
• Symbole du gène: SSSCA1
• Identification du gène (NCBI): 10534
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SSSCA1, also named as Autoantigen p27, is centromere-associated protein. It may induce anti-centromere antibodies. SSSCA1 plays a role in mitosis. It's involved in Sjogren syndrome/scleroderma. The calculated MW of SSSCA1 is 21.5 kDa (native form), with modification the MW is about23- 27 kDa.

Publications

Species	Application	Title
human	WB	Cell; Mechanism of orphan subunit recognition during assembly quality control; Authors - Yuichi Yagita ; KO Validated