Anticorps Polyclonal de lapin anti-ST6GAL1

• Phare
• Validé par KD/KO

ST6GAL1 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

11

Avis (5)

N° de cat : 14355-1-AP

Synonymes

Alpha 2,6 ST 1, B cell antigen CD75, CD75, Sialyltransferase 1, SIAT1, ST6Gal I, ST6GAL1, ST6GalI, ST6N

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Galerie de données de validation

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  WB analysis using 14355-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14355-1-AP (ST6GAL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14355-1-AP

  WB result of ST6GAL1 antibody (14355-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ST6GAL1 transfected HepG2 cells.

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  WB analysis of mouse spleen using 14355-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 14355-1-AP (ST6GAL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 14355-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14355-1-AP (ST6GAL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 14355-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14355-1-AP (ST6GAL1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HuH-7 using 14355-1-AP

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 14355-1-AP (ST6GAL1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 14355-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 14355-1-AP (ST6GAL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 14355-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14355-1-AP (ST6GAL1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 14355-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14355-1-AP (ST6GAL1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Raji using 14355-1-AP

  IP Result of anti-ST6GAL1 (IP:14355-1-AP, 4ug; Detection:14355-1-AP 1:600) with Raji cells lysate 2800ug.

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  IHC staining of human skin using 14355-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 14355-1-AP (ST6GAL1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin using 14355-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 14355-1-AP (ST6GAL1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human ovary tumor using 14355-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14355-1-AP (ST6GAL1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 14355-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14355-1-AP (ST6GAL1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 14355-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14355-1-AP (ST6GAL1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 14355-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14355-1-AP (ST6GAL1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of Raji using 14355-1-AP

  1X10^6 Raji cells were intracellularly stained with 0.2 ug Anti-Human ST6GAL1 (14355-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH .

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Applications testées

• Résultats positifs en WB: cellules Ramos, cellules HepG2, cellules HuH-7, cellules Jurkat, cellules Raji, cellules U-937, tissu hépatique de rat, tissu hépatique de souris, tissu splénique de souris
• Résultats positifs en IP: cellules Raji
• Résultats positifs en IHC: tissu cutané humain, tissu de cancer du côlon humain, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules Raji,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 9 publications below
• IHC: See 6 publications below
• IF: See 2 publications below

Informations sur le produit

14355-1-AP cible ST6GAL1 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ST6GAL1 Protéine recombinante Ag5705
• Nom complet: ST6 beta-galactosamide alpha-2,6-sialyltranferase 1
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 43-45 kDa, 50-70 kDa
• Numéro d’acquisition GenBank: BC040009
• Symbole du gène: ST6GAL1
• Identification du gène (NCBI): 6480
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ST6GAL1 (β-galactoside α-2-6 sialyl transferase1; also known as ST6N or CD75) is a sialyltransferase mediating the glycosylation of proteins and lipids to form functionally important glycoproteins and glycolipids in the Golgi compartment. It is principally expressed in liver, placenta and skeletal muscle. ST6GAL1 undergoes proteolytic process to generate soluble form from membrane form. Western blot analysis of human liver using this antibody detects both isoforms between 43-50 kDa. Higher molecular weight of bands around 50-70 kDa can also be observed with unknown reason. (PMID: 15049997)

Publications

Species	Application	Title
human,mouse	WB,IF	Immunity; Immune checkpoint therapy-elicited sialylation of IgG antibodies impairs antitumorigenic type I interferon responses in hepatocellular carcinoma; Authors - Rui-Qi Wu
human,mouse	WB,IHC	Int J Cancer; Modification of α2,6-sialylation mediates the invasiveness and tumorigenicity of non-small cell lung cancer cells in vitro and in vivo via Notch1/Hes1/MMPs pathway.; Authors - Qingmin Yuan ; KD Validated
human,mouse	IHC,IF	Mol Cell Proteomics; Integrated Systems Analysis of the Murine and Human Pancreatic Cancer Glycomes Reveals a Tumor-Promoting Role for ST6GAL1; Authors - Emma Kurz ; KO Validated
human	WB	Front Oncol; High-Risk HPV16 E6 Activates the cGMP/PKG Pathway Through Glycosyltransferase ST6GAL1 in Cervical Cancer Cells.; Authors - Jun Wang ; KD Validated
human	WB	Am J Cancer Res; α1,6-Fucosyltransferase (FUT8) regulates the cancer-promoting capacity of cancer-associated fibroblasts (CAFs) by modifying EGFR core fucosylation (CF) in non-small cell lung cancer (NSCLC).; Authors - Fengzhou Li
rat	WB	Int J Food Sci Nutr; Ellagic acid alleviates TNBS-induced intestinal barrier dysfunction by regulating mucin secretion and maintaining tight junction integrity in rats; Authors - Bo Peng

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Vanessa (Verified Customer) (01-12-2024)

1:1000 dilution of the primary antibody accompanied by the recommended blocking, washing and incubation steps resulted in crisp bands with no background.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Jurkat cells

FH

Maggie (Verified Customer) (11-15-2023)

Overnight incubation

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: PC3 cells

ST6GAL1 Antibody Immunofluorescence validation (1:200 dilution) in PC3 cells (Cat no:14355-1-AP)

FH

Faezeh (Verified Customer) (01-29-2023)

It showed two bands in A549 and PANC1 cell lines, we validated that top band is the correct one using siRNA against ST6GAL1.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

ST6GAL1 Antibody Western Blot validation (1:1000 dilution) in (Cat no:14355-1-AP)

FH

Dawn (Verified Customer) (02-22-2022)

It is difficult to find good antibodies for glycoslytransferase genes, but this is a good one. It works in multiple cell lines as well.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: A549 cells - lysate after miRNA transfection.

ST6GAL1 Antibody Western Blot validation (1:1000 dilution) in A549 cells - lysate after miRNA transfection. (Cat no:14355-1-AP)

FH

Patricia (Verified Customer) (02-25-2020)

Did not work- even for positive control

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Ovarian cancer